Evidence map›Paper›PMID 1348251›Full record

ArticleThe Journal of cell biology1992

Intracellular cyclic AMP not calcium, determines the direction of vesicle movement in melanophores: direct measurement by fluorescence ratio imaging.

P J Sammak, S R Adams, A T Harootunian, M Schliwa, R Y Tsien

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
3.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 126 citations in OpenAlex.

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  18. Regulated bidirectional motility of melanophore pigment granules along microtubules in vitro.Proceedings of the National Academy of Sciences of the United States of America · 1997
    Article
  19. Article
  20. Single cell Ca2+/cAMP cross-talk monitored by simultaneous Ca2+/cAMP fluorescence ratio imaging.Proceedings of the National Academy of Sciences of the United States of America · 1996
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

P J SammakDepartment of Pharmacology, School of Medicine, University of California, San Diego, LaJolla 02093-0647.
S R Adams
A T Harootunian
M Schliwa
R Y Tsien
University of California San Diego · US

Funding

High Resolution Genomic Analysis of Amplicon StructureR01CA090421 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ALBERTSON, DONNA G. · 2003 to 2007
$1.0M
NCI NIH HHS CA 090421NIGMS NIH HHS GM 16070NIGMS NIH HHS GM31004
6 · The paper itself

Abstract

Intracellular movement of vesiculated pigment granules in angelfish melanophores is regulated by a signalling pathway that triggers kinesin and dyneinlike microtubule motor proteins. We have tested the relative importance of intracellular Ca2+ ([Ca2+]i) vs cAMP ([cAMP]i) in the control of such motility by adrenergic agonists, using fluorescence ratio imaging and many ways to artificially stimulate or suppress signals in these pathways. Fura-2 imaging reported a [Ca2+]i elevation accompanying pigment aggregation, but this increase was not essential since movement was not induced with the calcium ionophore, ionomycin, nor was movement blocked when the increases were suppressed by withdrawal of extracellular Ca2+ or loading of intracellular BAPTA. The phosphatase inhibitor, okadaic acid, blocked aggregation and induced dispersion at concentrations that suggested that the protein phosphatase PP-1 or PP-2A was continuously turning phosphate over during intracellular motility. cAMP was monitored dynamically in single living cells by microinjecting cAMP-dependent kinase in which the catalytic and regulatory subunits were labeled with fluorescein and rhodamine respectively (Adams et al., 1991. Nature (Lond.). 349:694-697). Ratio imaging of F1CRhR showed that the alpha 2-adrenergic receptor-mediated aggregation was accompanied by a dose-dependent decrease in [cAMP]i. The decrease in [cAMP]i was both necessary and sufficient for aggregation, since cAMP analogs or microinjected free catalytic subunit of A kinase-blocked aggregation or caused dispersal, whereas the cAMP antagonist RpcAMPs or the microinjection of the specific kinase inhibitor PKI5-24 amide induced aggregation. Our conclusion that cAMP, not calcium, controls bidirectional microtubule dependent motility in melanophores might be relevant to other instances of non-muscle cell motility.

Indexed as

Adrenergic alpha-AntagonistsAdrenergic beta-AntagonistsAnimalsCalciumCarbacholCell AggregationCyclic AMPDigitoninEgtazic AcidEpinephrineEthers, CyclicFishesFluorescent DyesFura-2IonomycinKinetics1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acidAdrenergic alpha-AntagonistsAdrenergic beta-AntagonistsCalciumCarbacholCyclic AMPDigitoninEgtazic AcidEpinephrineEthers, CyclicFluorescent DyesFura-2fura-2-amIonomycinOkadaic AcidPhosphoprotein PhosphatasesProtein Kinases

Identifiers

PMID1348251
PMCPMC2289409
OpenAlexW1994298423

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.