ArticleMolecular and cellular biology1992
Drugs affecting microtubule dynamics increase alpha-tubulin mRNA accumulation via transcription in Tetrahymena thermophila.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- Determinants of cytoplasmic microtubule depolymerization during ciliogenesis inLife science alliance · 2024Article
- Secretome profiling ofFrontiers in oncology · 2023Article
- Effects of six pyrimidine analogs on the growth of Tetrahymena thermophila and their implications in pyrimidine metabolism.PloS one · 2023Article
- Alpha-Tubulin Acetylation inFrontiers in cellular and infection microbiology · 2021Article
- Selectively high efficacy of eribulin against high-grade invasive recurrent and/or metastatic squamous cell carcinoma of the head and neck.Oncology letters · 2019Article
- Article
- Mechanistic studies of semicarbazone triapine targeting human ribonucleotide reductase in vitro and in mammalian cells: tyrosyl radical quenching not involving reactive oxygen species.The Journal of biological chemistry · 2012Article
- A simple microscopy assay to teach the processes of phagocytosis and exocytosis.CBE life sciences education · 2012Article
- Heterogeneity in the sensitivity of microtubules of Giardia lamblia to the herbicide oryzalin.Parasitology research · 2010Article
- Microtubule-interacting drugs induce moderate and reversible damage to human bone marrow mesenchymal stem cells.Cell proliferation · 2009Article
- Katanin regulates dynamics of microtubules and biogenesis of motile cilia.The Journal of cell biology · 2007Article
- Review
- Molecular bases for sensitivity to tubulin-binding herbicides in green foxtail.Plant physiology · 2004Article
- Gene-specific signal transduction between microtubules and tubulin genes in Tetrahymena thermophila.Molecular and cellular biology · 1995Article
- Structure-function analysis of antimicrotubule dinitroanilines against promastigotes of the parasitic protozoan Leishmania mexicana.Antimicrobial agents and chemotherapy · 1993Article
- Rapid and Reversible High-Affinity Binding of the Dinitroaniline Herbicide Oryzalin to Tubulin from Zea mays L.Plant physiology · 1993Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In cultured mammalian cells, an increase in the amount of tubulin monomer due to treatment with a microtubule-depolymerizing agent results in a rapid decline in tubulin synthesis. This autoregulatory response is mediated through a posttranscriptional mechanism which decreases the stability of tubulin message with no change in transcriptional activity of tubulin genes. Conversely, treatment with a microtubule-polymerizing drug, such as taxol, results in a slight increase in the synthesis of tubulin. Surprisingly, we find that two microtubule-depolymerizing agents, colchicine and oryzalin, actually cause an increase in alpha-tubulin synthesis and alpha-tubulin message in starved Tetrahymena thermophila. This increase is paralleled by an increase in transcription of alpha-tubulin sequences measured by run-on transcription, while the half-life of tubulin message measured by decay in the presence of actinomycin D does not change appreciably. Treatment of starved cells with taxol also produces an increase in alpha-tubulin synthesis via an increase in message abundance due to an increase in transcription of the alpha-tubulin gene. These results indicate that tubulin synthesis in T. thermophila is regulated very differently than in cultured mammalian cells.
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