ArticleThe Journal of membrane biology1992
Na+/H(+)-exchange in A6 cells: polarity and vasopressin regulation.
Article in The Journal of membrane biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 15 citations in OpenAlex.
- Arginine Vasopressin Modulates Ion and Acid/Base Balance by Regulating Cell Numbers of Sodium Chloride Cotransporter and HInternational journal of molecular sciences · 2020Article
- Polarization of adenosine effects on intracellular pH in A6 renal epithelial cells.Molecular pharmacology · 1997Article
- Vasopressin-dependent control of basolateral Na/H-exchange in highly differentiated A6-cell monolayers.The Journal of membrane biology · 1993Article
- Structure/function studies of the epithelial isoforms of the mammalian Na+/H+ exchanger gene family.The Journal of membrane biology · 1993Review
- Polarized membrane movements in A6 kidney cells are regulated by aldosterone and vasopressin/vasotocin.The Journal of membrane biology · 1993Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analyzed the mechanism of Na(+)-dependent pHi recovery from an acid load in A6 cells (an amphibian distal nephron cell line) by using the intracellular pH indicator 2'7'-bis(2-carboxyethyl)5,6 carboxyfluorescein (BCECF) and single cell microspectrofluorometry. A6 cells were found to express Na+/H(+)-exchange activity only on the basolateral membrane: Na+/H(+)-exchange activity follows simple saturation kinetics with an apparent Km for Na+ of approximately 11 mM; it is inhibited in a competitive manner by ethylisopropylamiloride (EIPA). This Na+/H(+)-exchange activity is inhibited by pharmacological activation of protein kinase A (PKA) as well as of protein kinase C (PKC). Addition of arginine vasopressin (AVP) either at low (subnanomolar) or at high (micromolar) concentrations inhibits Na+/H(+)-exchange activity; AVP stimulates IP3 production at low concentrations, whereas much higher concentrations are required to stimulate cAMP formation. These findings suggest that in A6 cells (i) Na+/H(+)-exchange is located in the basolateral membrane and (ii) PKC activation (heralded by IP3 turnover) is likely to be the mediator of AVP action at low AVP concentrations.
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