ArticleMolecular and cellular biochemistry1992
Blockade of insulin sensitive steady-state R-type Ca2+ channel by PN 200-110 in heart and vascular smooth muscle.
Article in Molecular and cellular biochemistry, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- Pathophysiology of Prediabetes Hyperinsulinemia and Insulin Resistance in the Cardiovascular System.Biomedicines · 2025Review
- Morphological and Functional Remodeling of Vascular Endothelium in Cardiovascular Diseases.International journal of molecular sciences · 2023Review
- Insulin-Induced Cardiomyocytes Hypertrophy That Is Prevented by Taurine via β-alanine-Sensitive NaNutrients · 2021Article
- Hormonal regulation and implication of cell signaling in calcium transfer by placenta.Endocrine · 2001Review
- Taurine indirectly increases [Ca]i by inducing Ca2+ influx through the Na(+)-Ca2+ exchanger.Molecular and cellular biochemistry · 1998Article
- Endothelin-1 and insulin activate the steady-state voltage dependent R-type Ca2+ channel in aortic smooth muscle cells via a pertussis toxin and cholera toxin sensitive G-protein.Molecular and cellular biochemistry · 1998Article
- Increases of T-type Ca2+ current in heart cells of the cardiomyopathic hamster.Molecular and cellular biochemistry · 1997Article
- Early fetal like slow Na+ current in heart cells of cardiomyopathic hamster.Molecular and cellular biochemistry · 1997Article
- The use of confocal microscopy in the investigation of cell structure and function in the heart, vascular endothelium and smooth muscle cells.Molecular and cellular biochemistry · 1997Article
- Modulation of cytosolic and nuclear Ca2+ and Na+ transport by taurine in heart cells.Molecular and cellular biochemistry · 1997Article
- Binding and distribution of three prototype calcium channel blockers in perfused rat liver.Molecular and cellular biochemistry · 1997Article
- Implication of the nucleus in excitation contraction coupling of heart cells.Molecular and cellular biochemistry · 1996Article
- Hypoxia increases the activity of Ca(2+)-sensitive K+ channels in cat cerebral arterial muscle cell membranes.Pflugers Archiv : European journal of physiology · 1994Article
- Role of R-type calcium channels in the response of the perfused arterial and venous mesenteric vasculature of the rat to platelet-activating factor.British journal of pharmacology · 1994Article
- Signal transduction mechanism for the stimulation of the sarcolemmal Na(+)-Ca2+ exchanger by insulin.Molecular and cellular biochemistry · 1994Article
- Neurohormone D induces ionic current changes in cockroach central neurones.Journal of comparative physiology. A, Sensory, neural, and behavioral physiology · 1994Article
- PAF activation of a voltage-gated R-type Ca2+ channel in human and canine aortic endothelial cells.British journal of pharmacology · 1993Article
- Contraction and intracellular calcium-ion elevation of cultured human aortic smooth muscle cells by endothelin-1, vasoactive intestinal contractor (VIC) and the derivatives.In vitro cellular & developmental biology. AnimalArticle
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effect of high K+ concentration, insulin and the L-type Ca2+ channel blocker PN 200-110 on cytosolic intracellular free calcium ([Ca2+]i) was studied in single ventricular myocytes of 10-day-old embryonic chick heart, 20-week-old human fetus and rabbit aorta (VSM) single cells using the Ca(2+)-sensitive fluorescent dye, Fura-2 microfluorometry and digital imaging technique. Depolarization of the cell membrane of both heart and VSM cells with continuous superfusion of 30 mM [K+]o induced a rapid transient increase of [Ca2+]i that was followed by a sustained component. The early transient increase of [Ca2+]i by high [K+]o was blocked by the L-type calcium channel antagonist nifedipine. However, the sustained component was found to be insensitive to this drug. PN 200-110 another L-type Ca2+ blocker was found to decrease both the early transient and the sustained increase of [Ca2+]i induced by depolarization of the cell membrane with high [K+]o. Insulin at a concentration of 40 to 80 microU/ml only produced a sustained increase of [Ca2+]i that was blocked by PN 200-110 or by lowering the extracellular Ca2+ concentration with EGTA. The sustained increase of [Ca2+]i induced by high [K+]o or insulin was insensitive to metabolic inhibitors such as KCN and ouabain as well to the fast Na+ channel blocker, tetrodotoxin and to the increase of intracellular concentrations of cyclic nucleotides. Using the patch clamp technique, insulin did not affect the L-type Ca2+ current and the delayed outward K+ current. These results suggest that the early increase of [Ca2+]i during depolarization of the cell membrane of heart and VSM cells with high [K+]o is due to the opening and decay of an L-type Ca2+ channel. However, the sustained increase of [Ca2+]i during a sustained depolarization is due to the activation of a resting (R) Ca2+ channel that is insensitive to lowering [ATP]i and sensitive to insulin.
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