ArticleMolecular biology of the cell1992
Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.
Article in Molecular biology of the cell, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
60 citing papers in PubMed, 193 citations in OpenAlex.
- A distinct phase of cyclin B (Cdc13) nuclear export at mitotic entry inOpen biology · 2025Article
- Atypical cyclins: the extended family portrait.Cellular and molecular life sciences : CMLS · 2020Review
- Aberrant activation of cyclin A-CDK induces G2/M-phase checkpoint in human cells.Cell cycle (Georgetown, Tex.) · 2020Article
- Mechanisms of Mitotic Kinase Regulation: A Structural Perspective.Frontiers in cell and developmental biology · 2018Review
- Cell Cycle-independent Role of Cyclin D3 in Host Restriction of Influenza Virus Infection.The Journal of biological chemistry · 2017Article
- Establishment of an immortalized cell line derived from the prairie vole via lentivirus-mediated transduction of mutant cyclin-dependent kinase 4, cyclin D, and telomerase reverse transcriptase.Experimental animals · 2016Article
- Ser or Leu: structural snapshots of mistranslation in Candida albicans.Frontiers in molecular biosciences · 2014Review
- Brain-derived neurotrophic factor-dependent cdk1 inhibition prevents G2/M progression in differentiating tetraploid neurons.PloS one · 2013Article
- Inhibition of human cytomegalovirus immediate-early gene expression by cyclin A2-dependent kinase activity.Journal of virology · 2012Article
- Centrosomal localization of cyclins E and A: structural similarities and functional differences.Cell cycle (Georgetown, Tex.) · 2011Article
- Cyclin A promotes S-phase entry via interaction with the replication licensing factor Mcm7.Molecular and cellular biology · 2011Article
- The structure of P-TEFb (CDK9/cyclin T1), its complex with flavopiridol and regulation by phosphorylation.The EMBO journal · 2008Article
- Distinct sequence elements of cyclin B1 promote localization to chromatin, centrosomes, and kinetochores during mitosis.Molecular biology of the cell · 2007Article
- Article
- The APC/C inhibitor, Emi1, is essential for prevention of rereplication.Genes & development · 2007Article
- Cell cycle regulated D3-type cyclins form active complexes with plant-specific B-type cyclin-dependent kinase in vitro.Plant molecular biology · 2006Article
- Cyclin-dependent kinase activity is required for progesterone receptor function: novel role for cyclin A/Cdk2 as a progesterone receptor coactivator.Molecular and cellular biology · 2005Article
- Article
- Noncatalytic requirement for cyclin A-cdk2 in p27 turnover.Molecular and cellular biology · 2004Article
- Cyclin D and cdk4 are required for normal development beyond the blastula stage in sea urchin embryos.Molecular and cellular biology · 2002Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The binding of cyclin A to p34cdc2 and p32cdk2 and the protein kinase activity of the complexes has been measured by cell-free translation of the corresponding mRNA in extracts of frog eggs, followed by immunoprecipitation. A variety of mutant cyclin A molecules have been constructed and tested in this assay. Small deletions and point mutations of highly conserved residues in the 100-residue "cyclin box" abolish binding and activation of both p34cdc2 and p32cdk2. By contrast, large deletions at the N-terminus have no effect on kinase binding and activation, until they remove residues beyond 161, where the first conserved amino acids are found in all known examples of cyclin A. At the C-terminus, removal of 14 or more amino acids abolishes activity. We also demonstrate that deletion of, or point mutations, in the cyclin A homologue of the 10-residue "destruction box," previously described in cyclin B (Glotzer et al., 1991) abolish cyclin proteolysis at the transition from M-phase to interphase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.