ArticleThe Journal of cell biology1992
Antibodies against 70-kD heat shock cognate protein inhibit mediated nuclear import of karyophilic proteins.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 177 citations in OpenAlex.
- Prophylactic antineoplastic activity of Toxoplasma gondii RH derived antigen against ehrlich solid carcinoma with evidence of shared antigens by comparative immunoblotting.Infectious agents and cancer · 2023Article
- Identification of HSPA8 as an interacting partner of MAB21L2 and an important factor in eye development.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Article
- Heat shock protein Grp78/BiP/HspA5 binds directly to TDP-43 and mitigates toxicity associated with disease pathology.Scientific reports · 2022Article
- Molecular dynamics of estrogen-related receptors and their regulatory proteins: roles in transcriptional control for endocrine and metabolic signaling.Anatomical science international · 2022Review
- Blocking nuclear export of HSPA8 after heat shock stress severely alters cell survival.Scientific reports · 2018Article
- 70-kDa heat shock cognate protein hsc70 mediates calmodulin-dependent nuclear import of the sex-determining factor SRY.The Journal of biological chemistry · 2013Article
- The anti-inflammatory effects of heat shock protein 72 involve inhibition of high-mobility-group box 1 release and proinflammatory function in macrophages.Journal of immunology (Baltimore, Md. : 1950) · 2007Article
- The 70-kD heat shock cognate protein (hsc70) facilitates the nuclear export of the import receptors.The Journal of cell biology · 2005Article
- Pairs of Vp1 cysteine residues essential for simian virus 40 infection.Journal of virology · 2005Article
- Chloroplast protein import inhibition by a soluble factor from wheat germ lysate.Plant molecular biology · 2002Article
- Hsp70 regulates the interaction between the peroxisome targeting signal type 1 (PTS1)-receptor Pex5p and PTS1.The Biochemical journal · 2001Article
- Heat shock protein 90 and the nuclear transport of progesterone receptor.Cell stress & chaperones · 2001Article
- Essential role of voltage-dependent anion channel in various forms of apoptosis in mammalian cells.The Journal of cell biology · 2001Article
- Chaperones in cell cycle regulation and mitogenic signal transduction: a review.Cell proliferation · 2000Review
- Nucleocytoplasmic transport of macromolecules.Microbiology and molecular biology reviews : MMBR · 1997Review
- A role for HDJ-2/HSDJ in correcting subnuclear trafficking, transactivation, and transrepression defects of a glucocorticoid receptor zinc finger mutant.Molecular biology of the cell · 1997Article
- The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Heat shock disassembles the nucleolus and inhibits nuclear protein import and poly(A)+ RNA export.The EMBO journal · 1996Article
- In vivo nuclear transport kinetics in Saccharomyces cerevisiae: a role for heat shock protein 70 during targeting and translocation.The Journal of cell biology · 1996Article
- Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins.The Journal of cell biology · 1996Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, we found that anti-DDDED antibodies strongly inhibited in vivo nuclear transport of nuclear proteins and that these antibodies recognized a protein of 69 kD (p69) from rat liver nuclear envelopes that showed specific binding activities to the nuclear location sequences (NLSs) of nucleoplasmin and SV-40 large T-antigen. Here we identified this protein as the 70-kD heat shock cognate protein (hsc70) based on its mass, isoelectric point, cellular localization, and partial amino acid sequences. Competition studies indicated that the recombinant hsc70 expressed in Escherichia coli binds to transport competent SV-40 T-antigen NLS more strongly than to the point mutated transport incompetent mutant NLS. To investigate the possible involvement of hsc70 in nuclear transport, we examined the effect of anti-hsc70 rabbit antibodies on the nuclear accumulation of karyophilic proteins. When injected into the cytoplasm of tissue culture cells, anti-hsc70 strongly inhibited the nuclear import of nucleoplasmin, SV-40 T-antigen NLS bearing BSA and histone H1. In contrast, anti-hsc70 IgG did not prevent the diffusion of lysozyme or 17.4-kD FITC-dextran into the nuclei. After injection of these antibodies, cells continued RNA synthesis and were viable. These results indicate that hsc70 interacts with NLS-containing proteins in the cytoplasm before their nuclear import.
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