ArticleProceedings of the National Academy of Sciences of the United States of America1992
DdPK3, which plays essential roles during Dictyostelium development, encodes the catalytic subunit of cAMP-dependent protein kinase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Genome editing across Dictyostelia species enables comparative functional genetics of social amoebas.Scientific reports · 2026Article
- An integrated, cross-regulation pathway model involving activating/adaptive and feed-forward/feed-back loops for directed oscillatory cAMP signal-relay/response during the development ofFrontiers in cell and developmental biology · 2023Article
- mTORC1/AMPK responses define a core gene set for developmental cell fate switching.BMC biology · 2019Article
- Generation of deletions and precise point mutations in Dictyostelium discoideum using the CRISPR nickase.PloS one · 2019Article
- The transcription factor Spores Absent A is a PKA dependent inducer of Dictyostelium sporulation.Scientific reports · 2018Article
- Protein kinase A regulates the Ras, Rap1 and TORC2 pathways in response to the chemoattractant cAMP inJournal of cell science · 2017Article
- The ABC transporter, AbcB3, mediates cAMP export in D. discoideum development.Developmental biology · 2015Article
- A RabGAP regulates life-cycle duration via trimeric G-protein cascades in Dictyostelium discoideum.PloS one · 2013Article
- The induction of autophagy by mechanical stress.Autophagy · 2011Article
- Novel isoform of the Xenopus tropicalis PKA catalytic alpha subunit: An example of alternative splicing.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2010Article
- Nonadaptive regulation of ERK2 in Dictyostelium: implications for mechanisms of cAMP relay.Molecular biology of the cell · 2006Article
- Dictyostelium stress-activated protein kinase alpha, a novel stress-activated mitogen-activated protein kinase kinase kinase-like kinase, is important for the proper regulation of the cytoskeleton.Molecular biology of the cell · 2003Article
- Constitutively active protein kinase A disrupts motility and chemotaxis in Dictyostelium discoideum.Eukaryotic cell · 2003Article
- PRKX, a phylogenetically and functionally distinct cAMP-dependent protein kinase, activates renal epithelial cell migration and morphogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Role for YakA, cAMP, and protein kinase A in regulation of stress responses of Dictyostelium discoideum cells.Molecular biology of the cell · 2002Article
- CulB, a putative ubiquitin ligase subunit, regulates prestalk cell differentiation and morphogenesis in Dictyostelium spp.Eukaryotic cell · 2002Article
- Role of PKA in the timing of developmental events in Dictyostelium cells.Microbiology and molecular biology reviews : MMBR · 1998Review
- Spalten, a protein containing Galpha-protein-like and PP2C domains, is essential for cell-type differentiation in Dictyostelium.Genes & development · 1998Article
- Chemoattractants induce tyrosine phosphorylation of ERK2 in Dictyostelium discoideum by diverse signalling pathways.The Biochemical journal · 1997Article
- Genetic networks that regulate development in Dictyostelium cells.Microbiological reviews · 1996Review
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Abstract
We have previously reported the analysis of DdPK3, a developmentally regulated putative serine/threonine kinase that shares approximately 50% amino acid sequence identity with metazoan cAMP-dependent protein kinase A (PKA) and protein kinase C, within their catalytic domains. Cells in which the DdPK3 gene has been disrupted do not aggregate but they are able to induce aggregation-stage genes in response to cAMP pulses and the prestalk-specific ras gene DdrasD in response to high continuous levels of cAMP but will not induce prespore gene expression. In this report, we present conclusive evidence that DdPK3 encodes the catalytic subunit of the Dictyostelium PKA. DdPK3 null cells lack kinase activity that phosphorylates a PKA-specific substrate and is specifically inhibitable by recombinant cAMP-dependent protein kinase inhibitor. DdPK3 expressed in Escherichia coli has PKA activity that is inhibitable by protein kinase inhibitor. When Ddpk3 null cells are complemented with DdPK3 expressed from an actin promoter on an extrachromosomal vector (low copy number), PKA activity is restored and the cells proceed to the slug stage but will not culminate, suggesting that properly regulated PKA activity is essential for culmination. Moreover, overexpressing DdPK3 in wild-type cells on integrating vectors (high copy number) from either an actin or prespore-specific promoter results in accelerated development and the ability to form mature spores in monolayer culture in the presence of high cAMP, a developmental potential lacking in wild-type cells.
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