ArticlePflugers Archiv : European journal of physiology1992
Aldosterone actions on basolateral Na+/H+ exchange in Madin-Darby canine kidney cells.
Article in Pflugers Archiv : European journal of physiology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 43 citations in OpenAlex.
- Glomerular Mesangial Cell pH Homeostasis Mediates Mineralocorticoid Receptor-Induced Cell Proliferation.Biomedicines · 2021Article
- Rapid Action of Aldosterone on Protein Levels of Sodium-Hydrogen Exchangers and Protein Kinase C Beta Isoforms in Rat Kidney.International journal of endocrinology · 2017Article
- Genomic and rapid effects of aldosterone: what we know and do not know thus far.Heart failure reviews · 2017Review
- Aldosterone Upregulates Transient Receptor Potential Melastatin 7 (TRPM7).The Journal of biological chemistry · 2016Article
- Effect of aldosterone on the amplification of oncolytic vaccinia virus in human cancer lines.The Korean journal of hepatology · 2011Article
- Non-genomic action of the mineralocorticoid aldosterone on cytosolic sodium in cultured kidney cells.The Journal of physiology · 1998Article
- In vivo phosphorylation of the epithelial sodium channel.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Rapid activation of Na+/H+ exchange by aldosterone in renal epithelial cells requires Ca2+ and stimulation of a plasma membrane proton conductance.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Rapid activation of KATP channels by aldosterone in principal cells of frog skin.The Journal of physiology · 1996Article
- Transcriptional control of sodium transport in tight epithelial by adrenal steroids.The Journal of membrane biology · 1995Review
- Imaging nuclear pores of aldosterone-sensitive kidney cells by atomic force microscopy.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Vasopressin-dependent control of basolateral Na/H-exchange in highly differentiated A6-cell monolayers.The Journal of membrane biology · 1993Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent studies, there has been a re-evaluation of the polarity of Na+/H+ exchange in Madin-Darby canine kidney (MDCK) cells. This study was designed to examine aldosterone actions on basolaterally located Na+/H+ exchange of MDCK cell monolayers grown on permeant filter supports; pHi was analysed in the absence of bicarbonate by using the pH-sensitive fluorescent probe 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein. Pre-exposure of MDCK cells to aldosterone led within 10-20 min to an alkalization of pHi (approximately 0.3 pH unit); this effect is prevented by an addition of dimethylamiloride to the basolateral superfusate. Addition of aldosterone led to stimulation of the basolaterally located Na+/H+ exchange activity (Na(+)-dependent recovery from an acid load); this effect required preincubation (more then 3 min) and was observed at 0.1 nM aldosterone. Pre-exposure (15 min) of MDCK monolayers to phorbol 12-myristate 13-acetate also led to an activation of Na+/H+ exchange; pre-exposure to 8-bromo-cAMP led to inhibition of Na+/H+ exchange activity. An inhibitory effect of aldosterone was observed if Na+/H+ exchange activity was analysed in the presence of aldosterone; the highest inhibitory effects (20%-30%) occurred at concentrations of 5 nM and higher. Aldosterone-dependent inhibition does not require preincubation and is fully reversible; it was only observed at low (20 mM) but not at high Na+ concentrations (130 mM). The data suggest that aldosterone has an instantaneous inhibitory effect on basolaterally located Na+/H+ exchange activity under conditions of low Na+, but stimulates the rate of transport activity upon preincubation under conditions of physiological Na+ concentrations.
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