ArticleThe Journal of cell biology1992
Evidence for functional homology in the F-actin binding domains of gelsolin and alpha-actinin: implications for the requirements of severing and capping.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 155 citations in OpenAlex.
- Force-modulated structural landscape of the catch bonding F-actin crosslinker α-actinin-4.bioRxiv : the preprint server for biology · 2026Article
- RAB-10 cooperates with EHBP-1 to capture vesicular carriers during post-Golgi exocytic trafficking.The Journal of cell biology · 2025Article
- Structural and functional insights into α-actinin isoforms and their implications in cardiovascular disease.The Journal of general physiology · 2025Review
- Mechanism of actin filament severing and capping by gelsolin.Nature structural & molecular biology · 2025Article
- Molecular basis of F-actin regulation and sarcomere assembly via myotilin.PLoS biology · 2021Article
- The mechanism of activation of the actin binding protein EHBP1 by Rab8 family members.Nature communications · 2020Article
- Novel inter-domain CaJournal of muscle research and cell motility · 2020Review
- Article
- The Stress-Inducible Protein DRR1 Exerts Distinct Effects on Actin Dynamics.International journal of molecular sciences · 2018Article
- Dynamics of Dystrophin's Actin-Binding Domain.Biophysical journal · 2018Article
- Mammalian Plakins, Giant Cytolinkers: Versatile Biological Functions and Roles in Cancer.International journal of molecular sciences · 2018Review
- Structural basis for high-affinity actin binding revealed by a β-III-spectrin SCA5 missense mutation.Nature communications · 2017Article
- β-III-spectrin spinocerebellar ataxia type 5 mutation reveals a dominant cytoskeletal mechanism that underlies dendritic arborization.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The role of MACF1 in nervous system development and maintenance.Seminars in cell & developmental biology · 2017Review
- The Structurally Plastic CH2 Domain Is Linked to Distinct Functions of Fimbrins/Plastins.The Journal of biological chemistry · 2016Article
- Dynamic Regulation of α-Actinin's Calponin Homology Domains on F-Actin.Biophysical journal · 2016Article
- Isoforms, structures, and functions of versatile spectraplakin MACF1.BMB reports · 2016Review
- Polycystin-2 (TRPP2) Regulation by Ca(2+) Is Effected and Diversified by Actin-Binding Proteins.Biophysical journal · 2015Article
- Article
- Arg kinase-binding protein 2 (ArgBP2) interaction with α-actinin and actin stress fibers inhibits cell migration.The Journal of biological chemistry · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The F-actin binding domains of gelsolin and alpha-actinin compete for the same site on actin filaments with similar binding affinities. Both contain tandem repeats of approximately 125 amino acids, the first of which is shown to contain the actin-binding site. We have replaced the F-actin binding domain in the NH2-terminal half of gelsolin by that of alpha-actinin. The hybrid severs filaments almost as efficiently as does gelsolin or its NH2-terminal half, but unlike the latter, requires calcium ions. The hybrid binds two actin monomers and caps the barbed ends of filaments in the presence or absence of calcium. The cap produced by the hybrid binds with lower affinity than that of gelsolin and is not stable: It dissociates from filament ends with a half life of approximately 15 min. Although there is no extended sequence homology between these two different F-actin binding domains, our experiments show that they are functionally equivalent and provide new insights into the mechanism of microfilament severing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.