ArticleCurrent genetics1992
Identification of UAS elements and binding proteins necessary for derepression of Saccharomyces cerevisiae fructose-1,6-bisphosphatase.
Article in Current genetics, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Cyclic AMP can decrease expression of genes subject to catabolite repression in Saccharomyces cerevisiae.Journal of bacteriology · 1999Article
- Sip4, a Snf1 kinase-dependent transcriptional activator, binds to the carbon source-responsive element of gluconeogenic genes.The EMBO journal · 1998Article
- Yeast carbon catabolite repression.Microbiology and molecular biology reviews : MMBR · 1998Review
- FacB, the Aspergillus nidulans activator of acetate utilization genes, binds dissimilar DNA sequences.The EMBO journal · 1998Article
- A 27 kDa protein binds to a positive and a negative regulatory sequence in the promoter of the ICL1 gene from Saccharomyces cerevisiae.The Biochemical journal · 1998Article
- Glucose derepression of gluconeogenic enzymes in Saccharomyces cerevisiae correlates with phosphorylation of the gene activator Cat8p.Molecular and cellular biology · 1997Article
- Isolation and identification of genes activating UAS2-dependent ADH2 expression in Saccharomyces cerevisiae.Genetics · 1996Article
- Dual influence of the yeast Cat1p (Snf1p) protein kinase on carbon source-dependent transcriptional activation of gluconeogenic genes by the regulatory gene CAT8.Nucleic acids research · 1996Article
- CAT5, a new gene necessary for derepression of gluconeogenic enzymes in Saccharomyces cerevisiae.The EMBO journal · 1995Article
- CAT8, a new zinc cluster-encoding gene necessary for derepression of gluconeogenic enzymes in the yeast Saccharomyces cerevisiae.Molecular and cellular biology · 1995Article
- Identification and characterization of regulatory elements in the phosphoenolpyruvate carboxykinase gene PCK1 of Saccharomyces cerevisiae.Molecular & general genetics : MGG · 1995Article
- A carbon source-responsive promoter element necessary for activation of the isocitrate lyase gene ICL1 is common to genes of the gluconeogenic pathway in the yeast Saccharomyces cerevisiae.Molecular and cellular biology · 1994Article
Corrections and comments
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Authors and funding
7 authors.
Funding
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Abstract
Fructose-1,6-bisphosphatase is a key enzyme in gluconeogenesis and the FBP1 gene is not transcribed during growth with glucose. Genetic analysis indicated a positive regulation of FBP1 expression after exhaustion of glucose. By linker-deletion analysis, two upstream activation sites (UAS1 and UAS2) were localized and the respective UAS-binding factors (DAP I and DAP II for derepression activating protein) were identified by gel retardation. UAS1 and UAS2 span about 30 bp each, and are separated by approximately 30 bp. Both UAS sites act synergistically. Although UAS1 showed some similarities to the DNA-binding consensus for the general yeast activator Rap1, competition experiments and DEAE-chromatography proved that DAP I and Rap1 correspond to different proteins. Gel retardation by DAP I depended on carbon sources and did not occur in cells growing logarithmically with glucose, whereas a strong retardation signal was obtained with ethanol-grown cells. The present results suggest that DAP I and DAP II are the final regulatory elements for glucose derepression.
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