ArticleMolecular biology of the cell1992
Activation of 5-HT1A receptors expressed in NIH-3T3 cells induces focus formation and potentiates EGF effect on DNA synthesis.
Article in Molecular biology of the cell, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 39 citations in OpenAlex.
- The Role of Serotonin in Breast Cancer Stem Cells.Molecules (Basel, Switzerland) · 2021Review
- Antagonists of the serotonin receptor 5A target human breast tumor initiating cells.BMC cancer · 2020Article
- A spheroid toxicity assay using magnetic 3D bioprinting and real-time mobile device-based imaging.Scientific reports · 2015Article
- Fluoxetine dose and administration method differentially affect hippocampal plasticity in adult female rats.Neural plasticity · 2014Article
- Serotonin depletion hampers survival and proliferation in neurospheres derived from adult neural stem cells.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2010Article
- Stem cell approaches in psychiatry--challenges and opportunities.Dialogues in clinical neuroscience · 2009Review
- Towards a quantitative representation of the cell signaling mechanisms of hallucinogens: measurement and mathematical modeling of 5-HT1A and 5-HT2A receptor-mediated ERK1/2 activation.Neuropharmacology · 2009Article
- Norepinephrine-mediated regulation of 5HT1 receptor functioning in human platelets.Neurochemical research · 2008Article
- Hippocampal neurogenesis: regulation by stress and antidepressants.Biological psychiatry · 2006Review
- The recombinant 5-HT1A receptor: G protein coupling and signalling pathways.British journal of pharmacology · 1999Review
- Promotion of cell growth by stimulation of cloned human 5-HT1D receptor sites in transfected C6-glial cells is highly sensitive to intrinsic activity at 5-HT1D receptors.Naunyn-Schmiedeberg's archives of pharmacology · 1996Article
- Pharmacology of cloned human 5-HT1D receptor-mediated functional responses in stably transfected rat C6-glial cell lines: further evidence differentiating human 5-HT1D and 5-HT1B receptors.Naunyn-Schmiedeberg's archives of pharmacology · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NIH-3T3 fibroblasts have been transfected with human serotonin 5-HT1A receptors. Clonal cell lines expressed between 40 and 500 fmol receptor/mg. 5-HT1A agonists strongly inhibited nonstimulated- as well as forskolin- or isoproterenol-stimulated adenylyl cyclase. The effects of 5-HT1A receptor activation on cell growth were investigated. 5-HT1A agonists accelerated cell division, generated foci, and increased DNA synthesis. The stimulation of [3H]thymidine incorporation was much stronger when tyrosine kinase receptors were activated concomitantly. Cyclic AMP (cAMP) elevating agents inhibited DNA synthesis induced by all mitogens tested. The mitogenic activity of 5-HT1A agonists did not seem to be linked to adenylyl cyclase inhibition because 1) we were not able to measure any decrease in intracellular cAMP levels under the conditions of DNA synthesis assay and 2) 2',5'-dideoxyadenosine, which strongly inhibited adenylyl cyclase, was not mitogenic and did not modify the mitogenic effects of 5-HT1A agonists. Pertussis toxin completely blocked potentiation of epidermal growth factor effect induced by 8-hydroxy-di-(n-propyl)aminotetralin, a 5-HT1A agonist, but only partially blocked the one induced by insulin. In conclusion, in transfected NIH-3T3 cells, transforming and mitogenic effects of 5-HT1A agonists involve a pertussis toxin-sensitive G protein but do not seem to be linked to adenylyl cyclase inhibition.
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