ArticleNucleic acids research1992
Different E-box regulatory sequences are functionally distinct when placed within the context of the troponin I enhancer.
Article in Nucleic acids research, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
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- Spotting the Pattern: A Review on White Coat Color in the Domestic Horse.Animals : an open access journal from MDPI · 2024Review
- miR-206 enforces a slow muscle phenotype.Journal of cell science · 2020Article
- Functional studies of the Ciona intestinalis myogenic regulatory factor reveal conserved features of chordate myogenesis.Developmental biology · 2013Article
- Excitation-transcription coupling in skeletal muscle: the molecular pathways of exercise.Biological reviews of the Cambridge Philosophical Society · 2011Review
- Identification of RANBP16 and RANBP17 as novel interaction partners for the bHLH transcription factor E12.Journal of cellular biochemistry · 2010Article
- Genomic organization and evolution of immunoglobulin kappa gene enhancers and kappa deleting element in mammals.Molecular immunology · 2009Article
- De-phosphorylation of MyoD is linking nerve-evoked activity to fast myosin heavy chain expression in rodent adult skeletal muscle.The Journal of physiology · 2007Article
- Novel transcription regulatory elements in Caenorhabditis elegans muscle genes.Genome research · 2004Article
- RNA interference of achaete-scute homolog 1 in mouse prostate neuroendocrine cells reveals its gene targets and DNA binding sites.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The haplolethal region at the 16F gene cluster of Drosophila melanogaster: structure and function.Genetics · 1999Article
- Fiber-type-specific transcription of the troponin I slow gene is regulated by multiple elements.Molecular and cellular biology · 1999Article
- Identification of a novel slow-muscle-fiber enhancer binding protein, MusTRD1.Molecular and cellular biology · 1998Article
- Molecular mechanisms of myogenic coactivation by p300: direct interaction with the activation domain of MyoD and with the MADS box of MEF2C.Molecular and cellular biology · 1997Article
- Flanking sequences modulate the cell specificity of M-CAT elements.Molecular and cellular biology · 1996Article
- Casein kinase II increases the transcriptional activities of MRF4 and MyoD independently of their direct phosphorylation.Molecular and cellular biology · 1996Article
- MyoD and myogenin act on the chicken myosin light-chain 1 gene as distinct transcriptional factors.Molecular and cellular biology · 1993Article
- Transcriptional regulator Leu3 of Saccharomyces cerevisiae: separation of activator and repressor functions.Molecular and cellular biology · 1993Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Basic helix-loop-helix (bHLH) regulatory proteins are known to bind to a single DNA consensus sequence referred to as an E-box. The E-box is present in the regulatory elements of many developmentally controlled genes, including most muscle-specific genes such as troponin I (TnI). Although the E-box consensus is minimally defined as CANNTG, the adjacent nucleotides of functional E-boxes are variable for genes regulated by the bHLH proteins. In order to examine how E-box regulatory regions containing different internal and flanking nucleotides function when placed within the context of a single regulatory element, the E-box region (14 bp) present within the TnI enhancer was substituted with the corresponding E-box sequences derived from the muscle-specific M-creatine kinase (MCK) and cardiac alpha-actin regulatory elements as well as from the immunoglobulin kappa (Ig kappa) enhancer. Within the TnI enhancer, the E-box sequence derived from cardiac alpha-actin was inactive whereas the corresponding sequence from the MCK right E-box efficiently restored wild-type enhancer activity in muscle cells. Intermediate levels of gene activity were observed for TnI enhancers containing E-boxes derived from the MCK left E-box site or from the Ig kappa E2 E-box. DNA binding studies of MyoD:E12 protein complexes with each substituted TnI enhancer confirmed that DNA binding activity in vitro mimics the relative strength of the enhancers in vivo. These studies demonstrate that the specific nucleotide composition of individual E-boxes, which are contained within the regulatory elements of most if not all muscle-specific genes, contributes to the complex regulatory mechanisms governing bHLH-mediated gene expression.
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