ArticleMolecular and cellular biology1992
Jun is phosphorylated by several protein kinases at the same sites that are modified in serum-stimulated fibroblasts.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 123 citations in OpenAlex.
- AP1 Transcription Factor in the Regulation of the Urokinase Plasminogen Activation System.Biomolecules · 2026Review
- JUNB and JUND in Urological Cancers: A Literature Review.Current issues in molecular biology · 2025Review
- Impact of c-JUN deficiency on thalamus development in mice and human neural models.Cell & bioscience · 2024Article
- Comparative RNA-Seq transcriptome analyses reveal dynamic time-dependent effects ofBMC genomics · 2020Article
- Review
- Mechanosensing by the α6-integrin confers an invasive fibroblast phenotype and mediates lung fibrosis.Nature communications · 2016Article
- Endogenous interleukin-4 regulates glutathione synthesis following acetaminophen-induced liver injury in mice.Chemical research in toxicology · 2012Article
- c-Jun, at the crossroad of the signaling network.Protein & cell · 2011Review
- ERK-associated changes of AP-1 proteins during fear extinction.Molecular and cellular neurosciences · 2011Article
- Repeated probing of Southwestern blots using alkaline phosphatase stripping.Journal of chromatography. A · 2010Article
- Role of basic leucine zipper proteins in transcriptional regulation of the steroidogenic acute regulatory protein gene.Molecular and cellular endocrinology · 2009Review
- CAPERalpha is a novel Rel-TAD-interacting factor that inhibits lymphocyte transformation by the potent Rel/NF-kappaB oncoprotein v-Rel.Journal of virology · 2008Article
- Purification and identification of positive regulators binding to a novel element in the c-Jun promoter.Biochemistry · 2008Article
- The functional contrariety of JNK.Molecular carcinogenesis · 2007Review
- Pathway interactions between MAPKs, mTOR, PKA, and the glucocorticoid receptor in lymphoid cells.Cancer cell international · 2007Article
- COOH-terminal Src kinase-mediated c-Jun phosphorylation promotes c-Jun degradation and inhibits cell transformation.Cancer research · 2006Article
- NF-kappa B-dependent assembly of an enhanceosome-like complex on the promoter region of apoptosis inhibitor Bfl-1/A1.Molecular and cellular biology · 2003Article
- The response of c-jun/AP-1 to chronic hypoxia is hypoxia-inducible factor 1 alpha dependent.Molecular and cellular biology · 2002Article
- Cellular N-Ras promotes cell survival by downregulation of Jun N-terminal protein kinase and p38.Molecular and cellular biology · 2002Article
- Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
c-jun is a member of the family of immediate-early genes whose expression is induced by factors such as serum stimulation, phorbol ester, and differentiation signals. Here we show that increased Jun synthesis after serum stimulation is accompanied by a concomitant increase in phosphorylation. Several serine-threonine kinases were evaluated for their ability to phosphorylate Jun in vitro. p34cdc2, protein kinase C, casein kinase II, and pp44mapk phosphorylated Jun efficiently, whereas cyclic AMP-dependent protein kinase and glycogen synthase kinase III did not. The sites phosphorylated by p34cdc2 were similar to those phosphorylated in vivo after serum induction. The major sites of phosphorylation were mapped to serines 63, 73, and 246. Phosphorylation of full-length Jun with several kinases did not affect the DNA-binding activity of Jun homodimers or Fos-Jun heterodimers. Comparison of the DNA binding and in vitro transcription properties of wild-type and mutated proteins containing either alanine or aspartic acid residues in place of Ser-63, -73, and -246 revealed only minor differences among homodimeric complexes and no differences among Fos-Jun heterodimers. Thus, phosphorylation of Jun did not produce a significant change in dimerization, DNA-binding, or in vitro transcription activity. The regulatory role of phosphorylation in the modulation of Jun function is likely to be considerably more complex than previously suggested.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.