ArticleJournal of clinical pathology1992
Epstein Barr virus (EBV) encoded small RNAs: targets for detection by in situ hybridisation with oligonucleotide probes.
Article in Journal of clinical pathology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 107 citations in OpenAlex.
- Advances in Epstein-Barr Virus Detection: From Traditional Methods to Modern Technologies.Viruses · 2025Review
- Epstein-Barr virus modulates iron metabolism and ferritin expression to promote tumorigenesis in gastric cancer.Journal of molecular histology · 2025Article
- The CRISPR-Cas13a Gemini System for noncontiguous target RNA activation.Nature communications · 2024Article
- Assessing the Efficacy of VLP-Based Vaccine against Epstein-Barr Virus Using a Rabbit Model.Vaccines · 2023Article
- The Impact of Deleting Stem-Loop 1 of Epstein-Barr Virus-Encoded RNA 1 on Cell Proliferation.Viruses · 2022Article
- Virus-Driven Carcinogenesis.Cancers · 2021Review
- Review
- Tree Shrew Is a Suitable Animal Model for the Study of Epstein Barr Virus.Frontiers in immunology · 2021Article
- State of the Art and Future Challenges in Multiple Sclerosis Research and Medical Management: An Insight into the 5th International Porto Congress of Multiple Sclerosis.Neurology and therapy · 2020Review
- Recent Advances in Diagnostic Approaches for Epstein-Barr Virus.Pathogens (Basel, Switzerland) · 2020Review
- Tracking EBV-encoded RNAs (EBERs) from the nucleus to the excreted exosomes of B-lymphocytes.Scientific reports · 2018Article
- Hemophagocytic lymphohistiocytosis presenting with annular erythema multiforme-like eruptions in a patient with angioimmunoblastic T cell lymphoma: A case report.Experimental and therapeutic medicine · 2018Article
- Molecular signature of Epstein-Barr virus infection in MS brain lesions.Neurology(R) neuroimmunology & neuroinflammation · 2018Article
- A study of Epstein-Barr virus infection in the Chinese tree shrew(Tupaia belangeri chinensis).Virology journal · 2017Article
- Filaggrin gene polymorphism associated with Epstein-Barr virus-associated tumors in China.Virus genes · 2017Article
- A paper-based platform for detection of viral RNA.The Analyst · 2017Article
- No evidence for the presence of Epstein-Barr virus in squamous cell carcinoma of the mobile tongue.PloS one · 2017Article
- Sequence analysis of Epstein-Barr virus (EBV) early genes BARF1 and BHRF1 in NK/T cell lymphoma from Northern China.Virology journal · 2015Article
- Healthy rabbits are susceptible to Epstein-Barr virus infection and infected cells proliferate in immunosuppressed animals.Virology journal · 2015Article
- Sequence Variation Analysis of Epstein-Barr Virus Nuclear Antigen 1 Gene in the Virus Associated Lymphomas of Northern China.PloS one · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo develop a rapid, sensitive, and specific non-isotopic in situ hybridisation (NISH) procedure for the detection of Epstein-Barr virus in formalin fixed, paraffin wax embedded tissues.
methodsTwo low molecular weight RNAs, designated EBER-1 and EBER-2 (Epstein-Barr encoded RNA), were used: cells latently infected with EBV secrete large amounts of EBERs. The method uses digoxigenin labelled anti-sense oligonucleotides, corresponding to sequences in EBER-1 and EBER-2.
resultsThe use of these probes, in conjunction with high temperature microwave denaturation, ensured that the technique was considerably more sensitive than other in situ hybridisation techniques for detecting EBV. Furthermore, the hybridisation signal was morphologically distinct in that only the nucleus and not the nucleolus give a positive signal. No cross-hybridisation was observed with cells infected with other lymphotropic herpes viruses.
conclusionThe sensitivity, simplicity, and rapidity of this technique make it ideal for diagnostic use, and for studies investigating the role of this virus in neoplastic disease.
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