ArticleThe American journal of pathology1992
Autoantibodies developing to myeloperoxidase and proteinase 3 in systemic vasculitis stimulate neutrophil cytotoxicity toward cultured endothelial cells.
Article in The American journal of pathology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
72 citing papers in PubMed, 287 citations in OpenAlex.
- Targeting FcRn for immunomodulation: a promising therapy in autoimmune inflammatory rheumatic diseases.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026Review
- Anti-myeloperoxidase IgM B cells in anti-neutrophil cytoplasmic antibody-associated vasculitis.Nature communications · 2025Article
- Cerebrovascular involvement in systemic childhood vasculitides.Clinical rheumatology · 2023Review
- Genetics of ANCA-associated vasculitis: role in pathogenesis, classification and management.Nature reviews. Rheumatology · 2022Review
- Ruptured arterial aneurysm in Wegener's granulomatosis: a case report.Journal of medical case reports · 2021Article
- Article
- Pathogenicity of Proteinase 3-Anti-Neutrophil Cytoplasmic Antibody in Granulomatosis With Polyangiitis: Implications as Biomarker and Future Therapies.Frontiers in immunology · 2021Review
- Endothelial dysfunction in patients with granulomatosis with polyangiitis: a case-control study.Rheumatology international · 2018Article
- Pathogenesis of ANCA-Associated Pulmonary Vasculitis.Seminars in respiratory and critical care medicine · 2018Review
- Accessory left gastric artery aneurysms in granulomatosis with polyangiitis: a case report and literature review.Nagoya journal of medical science · 2017Review
- Induction of proteinase 3-anti-neutrophil cytoplasmic autoantibodies by proteinase 3-homologous bacterial protease in mice.Immunologic research · 2016Article
- Benefits of an expanded use of plasma exchange for anti-neutrophil cytoplasmic antibody-associated vasculitis within a dedicated clinical service.BMC musculoskeletal disorders · 2015Article
- Comparative investigation of respiratory tract involvement in granulomatosis with polyangiitis between PR3-ANCA positive and MPO-ANCA positive cases: a retrospective cohort study.BMC pulmonary medicine · 2015Article
- The immunopathology of ANCA-associated vasculitis.Seminars in immunopathology · 2014Review
- Current and future prospects in the management of granulomatosis with polyangiitis (Wegener's granulomatosis).Therapeutics and clinical risk management · 2014Review
- Pathogenesis of antineutrophil cytoplasmic autoantibody-associated small-vessel vasculitis.Annual review of pathology · 2013Review
- Epitope specificity of myeloperoxidase antibodies: identification of candidate human immunodominant epitopes.Clinical and experimental immunology · 2011Article
- Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation.The Journal of biological chemistry · 2011Article
- In vivo approaches to investigate ANCA-associated vasculitis: lessons and limitations.Arthritis research & therapy · 2011Review
- Myeloperoxidase-specific plasma cell depletion by bortezomib protects from anti-neutrophil cytoplasmic autoantibodies-induced glomerulonephritis.Journal of the American Society of Nephrology : JASN · 2011Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of vasculitis-associated anti-neutrophil cytoplasm antibodies (ANCA) to activate neutrophils and mediate release of radiolabel from 111Indium-labeled cultured human umbilical vein endothelial cells (HUVEC) was determined as a measure of the potential cytotoxicity of ANCA-activated neutrophils against vascular endothelium. Priming of neutrophils with low doses of phorbol 12-myristate 13-acetate (PMA) (1 ng/ml) and ionomycin (0.1 mumol/1) was required, together with pretreatment of endothelial cells with BCNU (1,3-bis-[2-chloroethyl]-1-nitrosourea; 0.26 mmol/l). Under these conditions and using a 4-hour serum-free assay system, mouse monoclonal antibodies (MAb) to the target autoantigens proteinase-3 (Pr-3) and myeloperoxidase (MPO) mediated enhanced release of 111Indium from HUVEC compared with control MAb. Human IgG Fab2 C-ANCA (recognizing Pr-3) and P-ANCA (recognizing MPO) did likewise. Preactivation of HUVEC with TNF (50 U/ml, 4 hr) enhanced the release of 111Indium from HUVEC generated by neutrophils activated with anti-Pr-3 and anti-MPO MAb. These data support the suggestion that activation of neutrophils by ANCA within the vascular lumen may contribute to endothelial cell injury.
Indexed as
Identifiers
1323218PMC1886603W143501003What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.