ArticleMolecular and cellular biology1992
Microinjection of smg/rap1/Krev-1 p21 into Swiss 3T3 cells induces DNA synthesis and morphological changes.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 105 citations in OpenAlex.
- SmgGDS: An Emerging Master Regulator of Prenylation and Trafficking by Small GTPases in the Ras and Rho Families.Frontiers in molecular biosciences · 2021Review
- Mutated RAP1GDS1 causes a new syndrome of dysmorphic feature, intellectual disability & speech delay.Annals of clinical and translational neurology · 2020Article
- The chaperone protein SmgGDS interacts with small GTPases entering the prenylation pathway by recognizing the last amino acid in the CAAX motif.The Journal of biological chemistry · 2014Article
- SmgGDS-558 regulates the cell cycle in pancreatic, non-small cell lung, and breast cancers.Cell cycle (Georgetown, Tex.) · 2014Article
- Article
- SmgGDS antagonizes BPGAP1-induced Ras/ERK activation and neuritogenesis in PC12 cell differentiation.Molecular biology of the cell · 2013Article
- Rap1 stabilizes beta-catenin and enhances beta-catenin-dependent transcription and invasion in squamous cell carcinoma of the head and neck.Clinical cancer research : an official journal of the American Association for Cancer Research · 2010Article
- Crk and CrkL adaptor proteins: networks for physiological and pathological signaling.Cell communication and signaling : CCS · 2009Article
- Spa-1 (Sipa1) and Rap signaling in leukemia and cancer metastasis.Cancer science · 2009Review
- Rap1GAP inhibits tumor growth in oropharyngeal squamous cell carcinoma.The American journal of pathology · 2006Article
- Mechanism of the spatio-temporal regulation of Ras and Rap1.The EMBO journal · 2003Article
- Drosophila PDZ-GEF, a guanine nucleotide exchange factor for Rap1 GTPase, reveals a novel upstream regulatory mechanism in the mitogen-activated protein kinase signaling pathway.Molecular and cellular biology · 2002Article
- Beyond the RING: CBL proteins as multivalent adapters.Oncogene · 2001Review
- Requirement for C3G-dependent Rap1 activation for cell adhesion and embryogenesis.The EMBO journal · 2001Article
- Cyclic AMP-mediated inhibition of cell growth requires the small G protein Rap1.Molecular and cellular biology · 2001Article
- TSC1 and TSC2 tumor suppressors antagonize insulin signaling in cell growth.Genes & development · 2001Article
- Human papillomavirus type 16 E6-induced degradation of E6TP1 correlates with its ability to immortalize human mammary epithelial cells.Journal of virology · 2001Article
- The Crk signaling pathway contributes to the bombesin-induced activation of the small GTPase Rap1 in Swiss 3T3 cells.Oncogene · 2000Article
- c-Src signaling induced by the adapters Sin and Cas is mediated by Rap1 GTPase.Molecular and cellular biology · 2000Article
- Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microinjection of either Ki-rasVal-12 p21 or the GDP-bound form of Ki-ras p21 plus smg GDP dissociation stimulator (GDS), a stimulatory GDP/GTP exchange protein for Ki-ras p21, smg/rap1/Krev-1 p21, and rho p21, into quiescent Swiss 3T3 cells induced DNA synthesis irrespective of the presence or absence of insulin. The guanosine 5'-(3-O-thio)triphosphate (GTP gamma S)-bound form of smg p21B or the GDP-bound form of smg p21B plus smg GDS also induced DNA synthesis but only in the presence of insulin. Either the GDP-bound form of Ki-ras p21 or the same form of smg p21B alone was inactive, but smg GDS alone was slightly active only in the presence of insulin. The morphology of the cells was analyzed by scanning electron, phase-contrast, and confocal laser scanning microscopies. Ki-rasVal-12 p21 induced membrane ruffling irrespective of the presence or absence of insulin. The GTP gamma S-bound form of smg p21B showed the same effect only in the presence of insulin. Either the GDP-bound form of Ki-ras p21, the same form of smg p21B, or smg GDS alone was inactive. Upon microinjection of Ki-rasVal-12 p21, stress fibers markedly decreased and the cells became round and piled up. In contrast, upon microinjection of the GTP gamma S-bound form of smg p21B, stress fibers did not markedly decrease and the cells neither became round nor piled up. These results indicate that both ras p21 and smg p21 are mitogenic in Swiss 3T3 cells but that their actions are slightly different.
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