ArticlePflugers Archiv : European journal of physiology1992
Polarized expression of Na+/H+ exchange activity in LLC-PK1/PKE20 cells: II. Hormonal regulation.
Article in Pflugers Archiv : European journal of physiology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 29 citations in OpenAlex.
- Glucose-induced regulation of NHEs activity and SGLTs expression involves the PKA signaling pathway.The Journal of membrane biology · 2011Article
- Cyclosporin A stimulates apical Na+/H+ exchange in LLC-PK1/PKE20 proximal tubular cells.Pediatric nephrology (Berlin, Germany) · 2006Article
- Calcitonin inhibits proton extrusion in resorbing rat osteoclasts via protein kinase A.Pflugers Archiv : European journal of physiology · 2003Article
- Polarization of adenosine effects on intracellular pH in A6 renal epithelial cells.Molecular pharmacology · 1997Article
- Basolateral membrane Na+/H+ exchange enhances HCO3- absorption in rat medullary thick ascending limb: evidence for functional coupling between basolateral and apical membrane Na+/H+ exchangers.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Expression of parathyroid hormone receptors in MDCK and LLC-PK1 cells.Pflugers Archiv : European journal of physiology · 1995Article
- The Na+/H+ exchanger: an update on structure, regulation and cardiac physiology.The Biochemical journal · 1993Review
- Na/H exchange activities in NHE1-transfected OK-cells: cell polarity and regulation.Pflugers Archiv : European journal of physiology · 1993Article
- Vasopressin-dependent control of basolateral Na/H-exchange in highly differentiated A6-cell monolayers.The Journal of membrane biology · 1993Article
- Structure/function studies of the epithelial isoforms of the mammalian Na+/H+ exchanger gene family.The Journal of membrane biology · 1993Review
- Apical and basolateral Na/H exchange in cultured murine proximal tubule cells (MCT): effect of parathyroid hormone (PTH).The Journal of membrane biology · 1992Article
- Na+/H(+)-exchange in A6 cells: polarity and vasopressin regulation.The Journal of membrane biology · 1992Article
- Aldosterone actions on basolateral Na+/H+ exchange in Madin-Darby canine kidney cells.Pflugers Archiv : European journal of physiology · 1992Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
LLC-PK1/PKE20 cells (a continuous epithelial cell line) has two different Na/H exchange activities: Na/H-1 located in the basolateral membrane and Na/H-2 located in the apical membrane [Casavola et al. (1989) Biochem Biophys Res Commun 165:833-837; Haggerty et al. (1988) Proc Natl Acad Sci USA 86:6797-6801]. In the present report we have studied hormone regulation of these exchange activities by measuring Na-dependent recovery of pHi from an acid load (by using microspectrofluorometry and 2,7-bis(carboxyethyl)-5,6-carboxyfluorescein) in response to activation of regulatory cascades by either pharmacological agents or by vasopressin or calcitonin. Agents leading to activation of protein kinase A (cAMP-dependent), such as forskolin (10 microM), 8-Br-cAMP (0.25 mM), and isobutylmethylxanthine (0.5 mM), inhibited Na/H-2 and Na/H-1 by an average of 49%. Stimulation of protein kinase C by a phorbol ester (phorbol 12-myristate 13-acetate, TPA, 100 nM) inhibited Na/H-2 (by an average of 48%) and stimulated Na/H-1 (by an average of 38%); these effects of TPA were also observed in the presence of forskolin (100 microM). Addition of either vasopressin (2 microM) or calcitonin (0.3 microM) onto both sides of the monolayer decreased the activity of Na/H-2 by an average of 26.3% and 27.7% respectively, and stimulated the activity of Na/H-1 by an average of 17.4% and 38.7% respectively; exposure of cells to either hormone stimulated production of cAMP and inositol trisphosphate, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
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