ArticleThe EMBO journal1992
The effect of tyrosine-specific protein phosphorylation on the assembly of adherens-type junctions.
Article in The EMBO journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 309 citations in OpenAlex.
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- Protein tyrosine phosphatases in cell adhesion.The Biochemical journal · 2021Review
- A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for β-catenin.Scientific reports · 2016Article
- Soluble adenylyl cyclase mediates hydrogen peroxide-induced changes in epithelial barrier function.Respiratory research · 2016Article
- Regulation of Endothelial Adherens Junctions by Tyrosine Phosphorylation.Mediators of inflammation · 2015Review
- Phosphatase regulation of intercellular junctions.Tissue barriers · 2013Review
- Neisseria gonorrhoeae breaches the apical junction of polarized epithelial cells for transmigration by activating EGFR.Cellular microbiology · 2013Article
- Prediction of functional phosphorylation sites by incorporating evolutionary information.Protein & cell · 2012Article
- Organotypic liver culture models: meeting current challenges in toxicity testing.Critical reviews in toxicology · 2012Review
- Pemphigus autoimmunity: hypotheses and realities.Autoimmunity · 2012Review
- Article
- Regulation of adherens junction dynamics by phosphorylation switches.Journal of signal transduction · 2012Article
- Receptor protein tyrosine phosphatases are novel components of a polycystin complex.Biochimica et biophysica acta · 2011Article
- Tissue organization by cadherin adhesion molecules: dynamic molecular and cellular mechanisms of morphogenetic regulation.Physiological reviews · 2011Review
- Phosphotyrosine protein dynamics in cell membrane rafts of sphingosine-1-phosphate-stimulated human endothelium: role in barrier enhancement.Cellular signalling · 2009Article
- The regulation of vascular endothelial growth factor-induced microvascular permeability requires Rac and reactive oxygen species.The Journal of biological chemistry · 2009Article
- Oxidative stress-induced disruption of epithelial and endothelial tight junctions.Frontiers in bioscience : a journal and virtual library · 2008Article
- Not so simple: the complexity of phosphotyrosine signaling at cadherin adhesive contacts.Journal of molecular medicine (Berlin, Germany) · 2007Review
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adherens-type junctions (AJs) are major subcellular targets for tyrosine specific protein phosphorylation [Volberg et al. (1991) Cell Regul., 2, 105-120]. Here we report on the apparent effect of such phosphorylation events on the assembly and integrity of AJs. We show that incubation of MDCK cells with potent inhibitors of tyrosine-specific phosphatases (PTP), namely H2O2 and vanadate, leads to a dramatic increase in AJ-associated phosphotyrosine which was apparent already within 2-5 min of treatment and progressed upon further incubation. Examination of H2O2 vanadate treated cells at later time points indicated that intercellular AJs rapidly deteriorated, concomitantly with a marked increase in the number and size of vinculin and actin containing focal contacts. In parallel, major changes were observed in cell structure and topology, as revealed by electron microscopy. These were manifested by rapid rounding-up of the cells followed by reorganization of the cell monolayer. Other intercellular junctions, including desmosomes and tight junctions, visualized by staining with desmoplakin and ZO-I antibodies, were not significantly affected. To verify that modulation of AJs was indeed related to tyrosine phosphorylation, we have carried out reciprocal experiments in which Rovs Sarcoma virus (RSV) transformed chick lens cells, expressing high levels of pp60src kinase, were treated with inhibitors of tyrosine kinases, (tyrphostins). We show that following such treatment, intercellular AJs which were deteriorated in the transformed cells, were reformed. Based on these observations, we propose that specific tyrosine phosphorylation of AJ components is involved in the downregulation of these cellular contacts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.