ArticleThe EMBO journal1992
Mutation of a cysteine in the first transmembrane segment of Na,K-ATPase alpha subunit confers ouabain resistance.
Article in The EMBO journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 108 citations in OpenAlex.
- Sensational site: the sodium pump ouabain-binding site and its ligands.American journal of physiology. Cell physiology · 2024Review
- Displacement of the NaProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Influence of Endogenous Cardiac Glycosides, Digoxin, and Marinobufagenin in the Physiology of Epithelial Cells.Cardiology research and practice · 2019Article
- On the effect of hyperaldosteronism-inducing mutations in Na/K pumps.The Journal of general physiology · 2017Article
- A structural rearrangement of the Na+/K+-ATPase traps ouabain within the external ion permeation pathway.Journal of molecular biology · 2015Article
- Route, mechanism, and implications of proton import during Na+/K+ exchange by native Na+/K+-ATPase pumps.The Journal of general physiology · 2014Article
- Ouabain modulates ciliogenesis in epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Ouabain binding site in a functioning Na+/K+ ATPase.The Journal of biological chemistry · 2011Article
- Na,K-ATPase is the putative membrane receptor of hormone ouabain.Communicative & integrative biology · 2010Article
- The two C-terminal tyrosines stabilize occluded Na/K pump conformations containing Na or K ions.The Journal of general physiology · 2010Article
- Review. Peering into an ATPase ion pump with single-channel recordings.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2009Review
- Article
- Roles of transmembrane segment M1 of Na+,K+-ATPase and Ca2-ATPase, the gatekeeper and the pivot.Journal of bioenergetics and biomembranes · 2007Review
- Ouabain affinity determining residues lie close to the Na/K pump ion pathway.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- A third Na+-binding site in the sodium pump.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The role of the third extracellular loop of the Na+,K+-ATPase alpha subunit in a luminal gating mechanism.The Journal of physiology · 2005Article
- Functional differences between alpha subunit isoforms of the rat Na,K-ATPase expressed in Xenopus oocytes.The Journal of physiology · 2002Article
- Structural similarities of Na,K-ATPase and SERCA, the Ca(2+)-ATPase of the sarcoplasmic reticulum.The Biochemical journal · 2001Review
- Structural insights into the binding of cardiac glycosides to the digitalis receptor revealed by solid-state NMR.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Does the colonic H,K-ATPase also act as an Na,K-ATPase?Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cardiac glycoside ouabain inhibits Na,K-ATPase by binding to the alpha subunit. In a highly ouabain resistant clone from the MDCK cell line, we have found two alleles of the alpha subunit in which the cysteine, present in the wild-type first transmembrane segment, is replaced by a tyrosine (Y) or a phenylalanine (F). We have studied the kinetics of ouabain inhibition by measuring the current generated by the Na,K-pump in Xenopus oocytes injected with wild-type and mutated alpha 1 and wild-type beta 1 subunit cRNAs. When these mutations, alpha 1C113Y and alpha 1C113F [according to the published sequence [Verrey et al. (1989) Am. J. Physiol., 256, F1034] were introduced in the alpha 1 subunit of the Na,K-ATPase from Xenopus laevis, the inhibition constant (Ki) of ouabain increased greater than 1000-fold compared with wild-type. A more conservative mutation, serine alpha 1C113S did not change the Ki. We observed that the decreased affinity for ouabain was mainly due to a faster dissociation, but probably also to a slower association. Thus we propose that an amino acid residue of the first transmembrane segment located deep in the plasma membrane participates in the structure and the function of the ouabain binding site.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.