ArticleThe Journal of cell biology1992
Proteins are secreted by both constitutive and regulated secretory pathways in lactating mouse mammary epithelial cells.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 89 citations in OpenAlex.
- Tebuconazole Induces ER-Stress-Mediated Cell Death in Bovine Mammary Epithelial Cell Lines.Toxics · 2023Article
- Occludin is a target of Src kinase and promotes lipid secretion by binding to BTN1a1 and XOR.PLoS biology · 2022Article
- Occludin protects secretory cells from ER stress by facilitating SNARE-dependent apical protein exocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- LUMAN/CREB3 Plays a Dual Role in Stress Responses as a Cofactor of the Glucocorticoid Receptor and a Regulator of Secretion.Frontiers in molecular neuroscience · 2018Article
- The endoplasmic reticulum and casein-containing vesicles contribute to milk fat globule membrane.Molecular biology of the cell · 2016Article
- Isolation of Endoplasmic Reticulum Fractions from Mammary Epithelial Tissue.Journal of mammary gland biology and neoplasia · 2016Article
- Astrocyte VAMP3 vesicles undergo Ca2+ -independent cycling and modulate glutamate transporter trafficking.The Journal of physiology · 2015Article
- Milk secretion: The role of SNARE proteins.Journal of mammary gland biology and neoplasia · 2014Review
- The membrane-associated form of α(s1)-casein interacts with cholesterol-rich detergent-resistant microdomains.PloS one · 2014Article
- Altered expression of tight junction proteins in mammary epithelium after discontinued suckling in mice.Pflugers Archiv : European journal of physiology · 2012Article
- The contribution of adhesion signaling to lactogenesis.Journal of cell communication and signaling · 2010Article
- Secretion and fluid transport mechanisms in the mammary gland: comparisons with the exocrine pancreas and the salivary gland.Journal of mammary gland biology and neoplasia · 2006Review
- Evidence of Rab3A expression, regulation of vesicle trafficking, and cellular secretion in response to heregulin in mammary epithelial cells.Molecular and cellular biology · 2000Article
- Secretion of milk proteins.Journal of mammary gland biology and neoplasia · 1998Review
- Origin and secretion of milk lipids.Journal of mammary gland biology and neoplasia · 1998Review
- Characterization of the effects of Ca2+ depletion on the synthesis, phosphorylation and secretion of caseins in lactating mammary epithelial cells.The Biochemical journal · 1996Article
- Feedback control of milk secretion from milk.Journal of mammary gland biology and neoplasia · 1996Review
- Molecular mechanisms in exocytosis.The Journal of membrane biology · 1995Review
- Regulation of milk lipid secretion: effects of oxytocin, prolactin and ionomycin on triacylglycerol release from rat mammary gland slices.The Biochemical journal · 1995Article
- Transforming growth factor-beta 1 inhibits casein secretion from differentiating mammary-gland explants but not from lactating mammary cells.The Biochemical journal · 1994Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactating mammary epithelial cells secrete high levels of caseins and other milk proteins. The extent to which protein secretion from these cells occurs in a regulated fashion was examined in experiments on secretory acini isolated from the mammary glands of lactating mice at 10 d postpartum. Protein synthesis and secretion were assayed by following the incorporation or release, respectively, of [35S]methionine-labeled TCA-precipitable protein. The isolated cells incorporated [35S]methionine into protein linearly for at least 5 h with no discernible lag period. In contrast, protein secretion was only detectable after a lag of approximately 1 h, consistent with exocytotic secretion of proteins immediately after passage through the secretory pathway and package into secretory vesicles. The extent of protein secretion was unaffected by the phorbol ester PMA, 8-bromo-cAMP, or 8-bromo-cGMP but was doubled by the Ca2+ ionophore ionomycin. In a pulse-label protocol in which proteins were prelabeled for 1 h before a chase period, constitutive secretion was unaffected by depletion of cytosolic Ca2+ but ionomycin was found to give a twofold stimulation of the secretion of presynthesized protein in a Ca(2+)-dependent manner. Ionomycin was still able to stimulate protein secretion after constitutive secretion had terminated. These results suggest that lactating mammary cells possess both a Ca(2+)-independent constitutive pathway and a Ca(2+)-activated regulatory pathway for protein secretion. The same proteins were secreted by both pathways. No ultrastructural evidence for apocrine secretion was seen in response to ionomycin and so it appears that regulated casein release involves exocytosis. Ionomycin was unlikely to be acting by disassembling the cortical actin network since cytochalasin D did not mimic its effects on secretion. The regulated pathway may be controlled by Ca2+ acting at a late step such as exocytotic membrane fusion.
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