ArticleRNA (New York, N.Y.)2003
B-cell and plasma-cell splicing differences: a potential role in regulated immunoglobulin RNA processing.
Article in RNA (New York, N.Y.), 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 56 citations in OpenAlex.
- Mystifying Molecular Structure, Expression and Repertoire Diversity of IgM Heavy Chain Genes (Frontiers in immunology · 2022Article
- A novel gene expression signature-based on B-cell proportion to predict prognosis of patients with lung adenocarcinoma.BMC cancer · 2021Article
- Cytoplasmic poly(A)-binding protein 1 (PABPC1) interacts with the RNA-binding protein hnRNPLL and thereby regulates immunoglobulin secretion in plasma cells.The Journal of biological chemistry · 2017Article
- Immunogenomic engineering of a plug-and-(dis)play hybridoma platform.Nature communications · 2016Article
- Coordination of RNA Polymerase II Pausing and 3' End Processing Factor Recruitment with Alternative Polyadenylation.Molecular and cellular biology · 2016Article
- A Lentiviral Vector Allowing Physiologically Regulated Membrane-anchored and Secreted Antibody Expression Depending on B-cell Maturation Status.Molecular therapy : the journal of the American Society of Gene Therapy · 2015Article
- B Cell-Intrinsic Expression of the HuR RNA-Binding Protein Is Required for the T Cell-Dependent Immune Response In Vivo.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- A Rapid Method to Characterize Mouse IgG Antibodies and Isolate Native Antigen Binding IgG B Cell Hybridomas.PloS one · 2015Article
- Transcription elongation factor ELL2 drives Ig secretory-specific mRNA production and the unfolded protein response.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Plasma cell formation, secretion, and persistence: the short and the long of it.Critical reviews in immunology · 2014Review
- Heterogeneous nuclear ribonucleoprotein L-like (hnRNPLL) and elongation factor, RNA polymerase II, 2 (ELL2) are regulators of mRNA processing in plasma cells.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Splicing of internal large exons is defined by novel cis-acting sequence elements.Nucleic acids research · 2012Article
- Characterization of a new ARID family transcription factor (Brightlike/ARID3C) that co-activates Bright/ARID3A-mediated immunoglobulin gene transcription.Molecular immunology · 2011Article
- Transcriptional and epigenetic regulation of B cell development.Immunologic research · 2011Review
- G-quadruplexes-novel mediators of gene function.Journal of cardiovascular translational research · 2011Review
- Transcription elongation factor ELL2 directs immunoglobulin secretion in plasma cells by stimulating altered RNA processing.Nature immunology · 2009Article
- Gene regulation by sense-antisense overlap of polyadenylation signals.RNA (New York, N.Y.) · 2009Article
- Control of the papillomavirus early-to-late switch by differentially expressed SRp20.Journal of virology · 2009Article
- GRSDB2 and GRS_UTRdb: databases of quadruplex forming G-rich sequences in pre-mRNAs and mRNAs.Nucleic acids research · 2008Article
- Widespread mRNA polyadenylation events in introns indicate dynamic interplay between polyadenylation and splicing.Genome research · 2007Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The immunoglobulin micro pre-mRNA is alternatively processed at its 3' end by competing splice and cleavage-polyadenylation reactions to generate mRNAs encoding the membrane-associated or secreted forms of the IgM protein, respectively. The relative use of the competing processing pathways varies during B-lymphocyte development, and it has been established previously that cleavage-polyadenylation activity is higher in plasma cells, which secrete IgM, than in B cells, which produce membrane-associated IgM. To determine whether RNA-splicing activity varies during B-lymphocyte development to contribute to micro RNA-processing regulation, we first demonstrate that micro pre-mRNA processing is sensitive to artificial changes in the splice environment by coexpressing SR proteins with the micro gene. To explore differences between the splice environments of B cells and plasma cells, we analyzed the splicing patterns from two different chimeric non-Ig genes that can be alternatively spliced but have no competing cleavage-polyadenylation reaction. The ratio of intact exon splicing to cryptic splice site use from one chimeric gene differs between several B-cell and several plasma-cell lines. Also, the amount of spliced RNA is higher in B-cell than plasma-cell lines from a set of genes whose splicing is dependent on a functional exonic splice enhancer. Thus, there is clear difference between the B-cell and plasma-cell splicing environments. We propose that both general cleavage-polyadenylation and general splice activities are modulated during B-lymphocyte development to ensure proper regulation of the alternative micro RNA processing pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.