ArticleJournal of bacteriology2003
Control of enzyme IIscr and sucrose-6-phosphate hydrolase activities in Streptococcus mutans by transcriptional repressor ScrR binding to the cis-active determinants of the scr regulon.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 15 citations in OpenAlex.
- Identification of novel fructo-oligosaccharide bacterial consumers by pulse metatranscriptomics in a human stool sample.mSphere · 2025Article
- Acid-adaptive mechanisms of Streptococcus mutans-the more we know, the more we don't.Molecular oral microbiology · 2017Review
- Comparative genomics of Clavibacter michiganensis subspecies, pathogens of important agricultural crops.PloS one · 2017Article
- Sucrose- and Fructose-Specific Effects on the Transcriptome of Streptococcus mutans, as Determined by RNA Sequencing.Applied and environmental microbiology · 2016Article
- The formation of Streptococcus mutans persisters induced by the quorum-sensing peptide pheromone is affected by the LexA regulator.Journal of bacteriology · 2015Article
- The malQ gene is essential for starch metabolism in Streptococcus mutans.Journal of oral microbiology · 2013Article
- A stress-inducible quorum-sensing peptide mediates the formation of persister cells with noninherited multidrug tolerance.Journal of bacteriology · 2012Article
- Distribution of putative virulence genes in Streptococcus mutans strains does not correlate with caries experience.Journal of clinical microbiology · 2011Article
- Streptococcus adherence and colonization.Microbiology and molecular biology reviews : MMBR · 2009Review
- Sucrose metabolism contributes to in vivo fitness of Streptococcus pneumoniae.Molecular microbiology · 2007Article
- A pleiotropic regulator, Frp, affects exopolysaccharide synthesis, biofilm formation, and competence development in Streptococcus mutans.Infection and immunity · 2006Article
- Inducible antisense RNA expression in the characterization of gene functions in Streptococcus mutans.Infection and immunity · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In Streptococcus mutans, enzyme II(scr) and sucrose-6-phosphate hydrolase are two important enzymes in the transport and metabolism of dietary sucrose. The scr regulon of S. mutans is composed of three genes, scrA and scrB, which code for enzyme II(scr) and sucrose-6-phosphate hydrolase, respectively, and scrR, which codes for a GalR-LacI-type transcription regulator. It was previously shown that expression of both scrA and scrB is similarly induced by sucrose. Mutation in the scrR gene resulted in increased expression of scrB relative to that in the wild-type strain. In this study, we employed DNA mobility shift and DNase I protection assays with a purified ScrR-histidine tag fusion protein to examine the DNA binding properties of ScrR to the promoter regions of the scrA and scrB genes. The results showed that ScrR bound specifically to the promoter regions of both scrA and scrB. Two regions with high affinity for ScrR in the promoter sequences of the scrA and scrB genes were identified by DNase I protection assays. One, O(C), which includes a 20-bp imperfect inverted-repeat sequence, is located between the two promoters, and the other, O(B), is located within the scrB promoter region containing a 37-bp imperfect direct-repeat sequence. Mutations of O(B) and O(C) resulted in constitutive transcription and expression of both the scrA and scrB genes. Our results indicated that S. mutans coordinates the activities of enzyme II(scr) and sucrose-6-phosphate hydrolase by transcriptional repressor ScrR binding to the promoter regions of the scr regulon.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.