ArticleJournal of bacteriology2003
Secretion of LamB-LacZ by the signal recognition particle pathway of Escherichia coli.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 68 citations in OpenAlex.
- Synonymous Codons and Hydrophobicity Optimization of Post-translational Signal Peptide PelB Increase Phage Display Efficiency of DARPins.ACS synthetic biology · 2022Article
- The biogenesis of β-lactamase enzymes.Microbiology (Reading, England) · 2022Review
- How Quality Control Systems AID Sec-Dependent Protein Translocation.Frontiers in molecular biosciences · 2021Review
- Trigger factor is a bona fide secretory pathway chaperone that interacts with SecB and the translocase.EMBO reports · 2020Article
- An experiment-informed signal transduction model for the role of the Staphylococcus aureus MecR1 protein in β-lactam resistance.Scientific reports · 2019Article
- Genetic Analysis of Protein Translocation.The protein journal · 2019Review
- The way is the goal: how SecA transports proteins across the cytoplasmic membrane in bacteria.FEMS microbiology letters · 2018Review
- Site-saturation mutagenesis of mutant L-asparaginase II signal peptide hydrophobic region for improved excretion of cyclodextrin glucanotransferase.Journal of industrial microbiology & biotechnology · 2017Article
- Article
- Enhancing full-length antibody production by signal peptide engineering.Microbial cell factories · 2016Article
- Folding LacZ in the periplasm of Escherichia coli.Journal of bacteriology · 2014Article
- Dominant negative lptE mutation that supports a role for LptE as a plug in the LptD barrel.Journal of bacteriology · 2013Article
- The Bam machine: a molecular cooper.Biochimica et biophysica acta · 2012Review
- Outer membrane biogenesis in Escherichia coli, Neisseria meningitidis, and Helicobacter pylori: paradigm deviations in H. pylori.Frontiers in cellular and infection microbiology · 2012Review
- Antibiotics as probes of biological complexity.Nature chemical biology · 2011Review
- Consequences of depletion of the signal recognition particle in Escherichia coli.The Journal of biological chemistry · 2011Article
- Transport of preproteins by the accessory Sec system requires a specific domain adjacent to the signal peptide.Journal of bacteriology · 2010Article
- Methionine oxidation contributes to bacterial killing by the myeloperoxidase system of neutrophils.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Effects of antibiotics and a proto-oncogene homolog on destruction of protein translocator SecY.Science (New York, N.Y.) · 2009Article
- Rational design of a fusion partner for membrane protein expression in E. coli.Protein science : a publication of the Protein Society · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
LamB-LacZ fusion proteins have classically been used in studies of the general secretion pathway of Escherichia coli. Here we describe how increasing signal sequence hydrophobicity routes LamB-LacZ Hyb42-1 to the signal recognition particle (SRP) pathway. Secretion of this hydrophobic fusion variant (H*LamB-LacZ) was reduced in the absence of fully functional Ffh and Ffs, and the translocator jamming caused by Hyb42-1 was prevented by efficient delivery of the fusion to the periplasm. Finally, we found that in the absence of the ribosome-associated chaperone, trigger factor (Tig), LamB-LacZ localized to the periplasm in a SecA-dependent, SRP-independent fashion. Collectively, our results provide compelling in vivo evidence that there is an SRP-dependent cotranslational targeting mechanism in E. coli and argue against a role for trigger factor in pathway discrimination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.