ArticleMolecular and cellular biology1992
Transcription of germ line V alpha segments correlates with ongoing T-cell receptor alpha-chain rearrangement.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Control of V(D)J Recombination through Transcriptional Elongation and Changes in Locus Chromatin Structure and Nuclear Organization.Genetics research international · 2011Article
- Interleukin 7 receptor control of T cell receptor gamma gene rearrangement: role of receptor-associated chains and locus accessibility.The Journal of experimental medicine · 1998Article
- Evidence that immunoglobulin VH-DJ recombination does not require germ line transcription of the recombining variable gene segment.Molecular and cellular biology · 1998Article
- The half-life of RAG-1 protein in precursor B cells is increased in the absence of RAG-2 expression.The Journal of experimental medicine · 1996Article
- Regulation of T cell receptor delta gene rearrangement by c-Myb.The Journal of experimental medicine · 1996Article
- The V(D)J recombinational and transcriptional activities of the immunoglobulin heavy-chain intronic enhancer can be mediated through distinct protein-binding sites in a transgenic substrate.Molecular and cellular biology · 1995Article
- Mouse T-cell receptor variable gene segment families.Immunogenetics · 1995Review
- Functional analysis of the V gamma 3 promoter of the murine gamma delta T-cell receptor.Molecular and cellular biology · 1994Article
- TCR beta and TCR alpha gene enhancers confer tissue- and stage-specificity on V(D)J recombination events.The EMBO journal · 1993Article
- Ordered rearrangement of variable region genes of the T cell receptor gamma locus correlates with transcription of the unrearranged genes.The Journal of experimental medicine · 1993Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
M14T is a virally transformed immature T-cell line which continues to rearrange its T-cell antigen receptor (TCR) alpha-chain genes in vitro and thus represents a dynamic system for studying TCR assembly. In an effort to investigate whether the TCR alpha locus is accessible for V(D)J rearrangement events, we examined M14T cells for the presence of germ line TCR alpha transcripts. Several unrearranged V alpha segments were found to be transcriptionally active in M14T cells. By comparison, germ line V alpha transcripts are absent in nonlymphoid and pro-T-cell lines and barely detectable in mature T-cell lines, suggesting that this phenomenon is likely stage and tissue specific. We demonstrate a perfect correlation between transcriptionally active V alpha segments and their involvement in ongoing V alpha-to-J alpha rearrangements. In addition, data suggesting that the unrearranged J alpha locus is also transcriptionally active in the M14T line are presented. Furthermore, the recombination-activating genes RAG-1 and RAG-2 are differentially expressed, with RAG-2 detectable only by polymerase chain reaction, implying that very low levels of one of these gene products are sufficient to complement the other to facilitate VJ rearrangements. These findings provide the first direct evidence for an accessibility model of antigen receptor rearrangement in T cells.
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