ArticleJournal of clinical microbiology1992
Comparison of the benzoyl-DL-arginine-naphthylamide (BANA) test, DNA probes, and immunological reagents for ability to detect anaerobic periodontal infections due to Porphyromonas gingivalis, Treponema denticola, and Bacteroides forsythus.
Article in Journal of clinical microbiology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 137 citations in OpenAlex.
- Random Mutagenesis in Porphyromonas gingivalis Enables Isolation of Mutants With Enhanced Secreted Protease Activity.Molecular oral microbiology · 2026Article
- Diagnosis in Periodontology and Implant Dentistry: The Past, the Present, the Future.Journal of periodontal research · 2026Article
- Article
- Measuring the Invisible: Microbial Diagnostics for Periodontitis-A Narrative Review.International journal of molecular sciences · 2025Review
- Evaluation of a Novel Immunochromatographic Device for DetectingInternational journal of molecular sciences · 2024Article
- Therapeutic effect of aged garlic extract on gingivitis in dogs.Frontiers in veterinary science · 2023Article
- The Ability of a Novel Trypsin-like Peptidase Activity Assay Kit to Detect Red-Complex Species.Diagnostics (Basel, Switzerland) · 2022Article
- A Preliminary Study on the Ability of the Trypsin-Like Peptidase Activity Assay Kit to Detect Periodontitis.Dentistry journal · 2020Article
- Red complex: Polymicrobial conglomerate in oral flora: A review.Journal of family medicine and primary care · 2019Review
- Saliva diagnostics - Current views and directions.Experimental biology and medicine (Maywood, N.J.) · 2017Review
- Mirolase, a novel subtilisin-like serine protease from the periodontopathogen Tannerella forsythia.Biological chemistry · 2015Article
- Quantitative molecular detection of putative periodontal pathogens in clinically healthy and periodontally diseased subjects.PloS one · 2014Article
- Periodontal proteomics: wonders never cease!International journal of proteomics · 2013Review
- Halitosis: From diagnosis to management.Journal of natural science, biology, and medicine · 2013Article
- Divergence of the systemic immune response following oral infection with distinct strains of Porphyromonas gingivalis.Molecular oral microbiology · 2012Article
- BANA-Positive Plaque Samples Are Associated with Oral Hygiene Practices and Not CD4+ T Cell Counts in HIV-Positive Patients.International journal of dentistry · 2012Article
- Highly specific protease-based approach for detection of porphyromonas gingivalis in diagnosis of periodontitis.Journal of clinical microbiology · 2012Article
- Role of periodontal pathogenic bacteria in RANKL-mediated bone destruction in periodontal disease.Journal of oral microbiology · 2010Article
- The relationship between physiologic halitosis and periodontopathic bacteria of the tongue and gingival sulcus.Odontology · 2010Article
- Oral fluid-based biomarkers of alveolar bone loss in periodontitis.Annals of the New York Academy of Sciences · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most forms of periodontal disease are associated with the presence or overgrowth of anaerobic species that could include Treponema denticola, Porphyromonas gingivalis, and Bacteroides forsythus among others. These three organisms are among the few cultivable plaque species that can hydrolyze the synthetic trypsin substrate benzoyl-DL-arginine-naphthylamide (BANA). In turn, BANA hydrolysis by the plaque can be associated with periodontal morbidity and with the presence of these three BANA-positive organisms in the plaque. In this investigation, the results of the BANA test, which simultaneously detects one or more of these organisms, were compared with the detection of these organisms by (i) highly specific antibodies to P. gingivalis, T. denticola, and B. forsythus; (ii) whole genomic DNA probes to P. gingivalis and T. denticola; and (iii) culturing or microscopic procedures. The BANA test, the DNA probes, and an enzyme-linked immunosorbent assay or an indirect immunofluorescence assay procedure exhibited high sensitivities, i.e., 90 ot 96%, and high accuracies, i.e., 83 to 92%, in their ability to detect combinations of these organisms in over 200 subgingival plaque samples taken from the most periodontally diseased sites in 67 patients. This indicated that if P. gingivalis, T. denticola, and B. forsythus are appropriate marker organisms for an anaerobic periodontal infection, then the three detection methods are equally accurate in their ability to diagnose this infection. The same statement could not be made for the culturing approach, where accuracies of 50 to 62% were observed.
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Registered trials
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