ArticleProceedings of the National Academy of Sciences of the United States of America1992
Immortalization of primary human smooth muscle cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 104 citations in OpenAlex.
- An Ex Vivo Model for the Onset of Human Labor.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Pancreatic Tumor Organoid-Derived Factors from Cachectic Patients Disrupt Contractile Smooth Muscle Cells.Cancers · 2024Article
- Tools, techniques, and future opportunities for characterizing the mechanobiology of uterine myometrium.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Article
- The expression of genes involved in myometrial contractility changes during ex situ culture of pregnant human uterine smooth muscle tissue.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2017Article
- Modulation of Progesterone Receptor Isoform Expression in Pregnant Human Myometrium.BioMed research international · 2017Article
- Surfactant Proteins SP-A and SP-D Modulate Uterine Contractile Events in ULTR Myometrial Cell Line.PloS one · 2015Article
- Development and validation of primary human myometrial cell culture models to study pregnancy and labour.BMC pregnancy and childbirth · 2013Article
- Fibronectin glycation increases IGF-I induced proliferation of human aortic smooth muscle cells.Diabetology & metabolic syndrome · 2012Article
- Arrestins differentially regulate histamine- and oxytocin-evoked phospholipase C and mitogen-activated protein kinase signalling in myometrial cells.British journal of pharmacology · 2011Article
- Long-chain acyl-CoA synthetase 4 modulates prostaglandin E₂ release from human arterial smooth muscle cells.Journal of lipid research · 2011Article
- Expression and distribution of NADPH oxidase isoforms in human myometrium--role in angiotensin II-induced hypertrophy.Biology of reproduction · 2010Article
- Characterization of anandamide-stimulated cannabinoid receptor signaling in human ULTR myometrial smooth muscle cells.Molecular endocrinology (Baltimore, Md.) · 2009Article
- Regulation of oxytocin receptor responsiveness by G protein-coupled receptor kinase 6 in human myometrial smooth muscle.Molecular endocrinology (Baltimore, Md.) · 2009Article
- Selective regulation of H1 histamine receptor signaling by G protein-coupled receptor kinase 2 in uterine smooth muscle cells.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Effect of serum replacement with plysate on cell growth and metabolismin primary cultures of human skeletal muscle.Cytotechnology · 2005Article
- Identification, structural, and functional characterization of a new early gene (6A3-5, 7 kb): implication in the proliferation and differentiation of smooth muscle cells.Journal of biomedicine & biotechnology · 2005Article
- Control of cellular cholesterol efflux by the nuclear oxysterol receptor LXR alpha.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Article
- A comparative molecular analysis of four rat smooth muscle cell lines.In vitro cellular & developmental biology. Animal · 1998Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Primary human aortic and myometrial smooth muscle cells (SMCs) were immortalized using an amphotropic recombinant retroviral construct containing the E6 and E7 open reading frames (ORFs) of human papillomavirus type 16. The SMCs expressing the E6/E7 ORFs have considerably elevated growth rates when compared with nonimmortalized control cells and show no signs of senescence with long-term passage. The first SMC line derived in this study has been maintained in continuous tissue culture for greater than 1 year (greater than 180 population doublings). The immortalized SMCs have decreased cell size and decreased content of muscle-specific alpha-actin filaments as determined by indirect immunofluorescence. Southern blot analysis has demonstrated the stable integration of the E6/E7 ORFs in the retrovirally infected cells, and radioimmunoprecipitation has confirmed the continued expression of the E6 and E7 genes. Cytogenetic studies of the SMC lines have revealed essentially diploid populations except for the myometrial clonal line, which became aneuploid at late passage (greater than 125 doublings). These cell lines were not tumorigenic in nude mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.