ArticleThe Journal of cell biology1992
In vivo analysis of functional domains from villin and gelsolin.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 46 citations in OpenAlex.
- Actin Bundles in The Pollen Tube.International journal of molecular sciences · 2018Review
- Tropomyosins Regulate the Severing Activity of Gelsolin in Isoform-Dependent and Independent Manners.Biophysical journal · 2018Article
- Interplay between Ions, the Cytoskeleton, and Cell Wall Properties during Tip Growth.Plant physiology · 2018Review
- DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells.Nature methods · 2017Article
- Helix straightening as an activation mechanism in the gelsolin superfamily of actin regulatory proteins.The Journal of biological chemistry · 2009Article
- The 3D structure of villin as an unusual F-Actin crosslinker.Structure (London, England : 1993) · 2008Article
- The isolated sixth gelsolin repeat and headpiece domain of villin bundle F-actin in the presence of calcium and are linked by a 40-residue unstructured sequence.Biochemistry · 2007Article
- Polyphosphoinositides-dependent regulation of the osteoclast actin cytoskeleton and bone resorption.BMC cell biology · 2004Article
- Gelsolin-induced epithelial cell invasion is dependent on Ras-Rac signaling.The EMBO journal · 2002Article
- Determination of the gelsolin binding site on F-actin: implications for severing and capping.Biophysical journal · 1998Article
- Refined structure of villin 14T and a detailed comparison with other actin-severing domains.Protein science : a publication of the Protein Society · 1997Article
- Molecular and mutational analysis of a gelsolin-family member encoded by the flightless I gene of Drosophila melanogaster.Genetics · 1995Article
- A gelsolin-related protein from lobster muscle: cloning, sequence analysis and expression.The Biochemical journal · 1995Article
- Autoantibodies specific for villin found in patients with colon cancer and other colitides.Digestive diseases and sciences · 1995Article
- Distribution of gelsolin in mouse ovary.Cell and tissue research · 1994Article
- Ezrin contains cytoskeleton and membrane binding domains accounting for its proposed role as a membrane-cytoskeletal linker.The Journal of cell biology · 1993Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transfected CV1 cells were used to compare the in vivo effects of various domains of villin and gelsolin. These two homologous actin modulating proteins both contain a duplicated severin-like sequence. Villin has in addition a carboxy-terminal domain, the headpiece, which accounts for its bundling activity. The effects of the villin-deleted mutants were compared with those of native villin. Our results show that essential domains of villin required to induce the growth of microvilli and F-actin redistribution are present in the first half of the core and in the headpiece. We also show that the second half of the villin core cannot be exchanged by its homolog in gelsolin. When expressed at high levels of CV1 cells, full length gelsolin completely disrupted stress fibers without change of the cell shape. Addition of the villin headpiece to gelsolin had no effect on the phenotype induced by gelsolin alone. Expression of the first half of gelsolin induced similar modifications as capping proteins and rapid cell mortality; this deleterious effect on the cell structure was also observed when the headpiece was linked to the first half of gelsolin. In cells expressing the second half of gelsolin, a dotted F-actin staining was often seen. Moreover elongated dorsal F-actin structures were observed when the headpiece was linked to the second gelsolin domain. These studies illustrate the patent in vivo severing activity of gelsolin as well as the distinct functional properties of villin core in contrast to gelsolin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.