ArticleJournal of virology1992
Identification of three transforming proteins encoded by cottontail rabbit papillomavirus.
Article in Journal of virology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 51 citations in OpenAlex.
- The full transcription map of cottontail rabbit papillomavirus in tumor tissues.PLoS pathogens · 2024Article
- Comparative structural studies on Bovine papillomavirus E6 oncoproteins: Novel insights into viral infection and cell transformation from homology modeling and molecular dynamics simulations.Genetics and molecular biology · 2024Article
- RNAi-Mediated Knockdown of Cottontail Rabbit Papillomavirus Oncogenes Using Low-Toxicity Lipopolyplexes as a Paradigm to Treat Papillomavirus-Associated Cancers.Pharmaceutics · 2023Article
- Review
- Modeling HPV-Associated Disease and Cancer Using the Cottontail Rabbit Papillomavirus.Viruses · 2022Review
- Article
- The rabbit papillomavirus model: a valuable tool to study viral-host interactions.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Comparison of human papillomavirus type 16 replication in tonsil and foreskin epithelia.Virology · 2016Article
- Review
- Papillomavirus E6 oncoproteins.Virology · 2013Review
- Inhibition of papilloma progression by antisense oligonucleotides targeted to HPV11 E6/E7 RNA.Gene therapy · 2004Article
- Amino acid residues in the carboxy-terminal region of cottontail rabbit papillomavirus E6 influence spontaneous regression of cutaneous papillomas.Journal of virology · 2002Article
- A transactivator function of cottontail rabbit papillomavirus e2 is essential for tumor induction in rabbits.Journal of virology · 2002Article
- Life cycle heterogeneity in animal models of human papillomavirus-associated disease.Journal of virology · 2002Article
- Ubiquitin-fused and/or multiple early genes from cottontail rabbit papillomavirus as DNA vaccines.Journal of virology · 2002Article
- Variation in the nucleotide sequence of cottontail rabbit papillomavirus a and b subtypes affects wart regression and malignant transformation and level of viral replication in domestic rabbits.Journal of virology · 2000Article
- DNA vaccination prevents and/or delays carcinoma development of papillomavirus-induced skin papillomas on rabbits.Journal of virology · 2000Article
- Granulocyte-macrophage colony-stimulating factor priming plus papillomavirus E6 DNA vaccination: effects on papilloma formation and regression in the cottontail rabbit papillomavirus--rabbit model.Journal of virology · 2000Article
- High efficiency, long-term clinical expression of cottontail rabbit papillomavirus (CRPV) DNA in rabbit skin following particle-mediated DNA transfer.Nucleic acids research · 1996Article
- Transforming properties of the cottontail rabbit papillomavirus oncoproteins Le6 and SE6 and of the E8 protein.Journal of virology · 1996Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cottontail rabbit papillomavirus (CRPV) provides an animal model for human papillomaviruses associated with a high risk of cancer development. So far, nothing is known about the transforming functions of CRPV genes because of the lack of an assay system. We have recently developed two systems to assay for CRPV transforming functions. One is based on the finding that transformation of NIH 3T3 cells by CRPV is considerably increased by deleting sequences in open reading frame L2. The second one is based on the use of a cottontail rabbit skin epithelial cell line, sf1Ep (C. Meyers and F. O. Wettstein, Virology 181:637-646, 1991). Mutations were introduced which abolished expression of the full-length E6 protein (LE6), the short E6 protein (SE6) initiated at the second ATG of E6, the E7 protein, or the E5 protein. Mutations affecting LE6 or E7, but not SE6, reduced transformation of NIH 3T3 and sf1Ep cells. Transformed NIH 3T3 cell lines with mutations in LE6 and E7 did not grow in soft agar, while those with mutations in SE6 and E5 grew with a reduced efficiency. The cell lines with mutations in LE6, SE6, or E7 still did induce tumors in nude mice. These mutations, however, abolished the ability to induce papillomas in rabbits. When expressed individually with a retroviral vector, LE6, SE6, or E7, but not E5, conferred anchorage-independent growth. The level of viral protein expression in these cell lines was generally low, and a comparison of the abundance of virus-specific mRNA showed that cell lines contained 20 to 50 times less mRNA than a cottontail rabbit papilloma. These data demonstrate that CRPV encodes at least three transforming proteins.
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