ArticleThe Journal of cell biology1992
Phorbol ester induced osteoclast-like differentiation of a novel human leukemic cell line (FLG 29.1).
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 85 citations in OpenAlex.
- Article
- Human bone marrow-derived MSCs can home to orthotopic breast cancer tumors and promote bone metastasis.Cancer research · 2010Article
- A novel osteoclast precursor cell line, 4B12, recapitulates the features of primary osteoclast differentiation and function: enhanced transfection efficiency before and after differentiation.Journal of cellular physiology · 2009Article
- Release of prostaglandin E(1) from N-(2-hydroxypropyl)methacrylamide copolymer conjugates by bone cells.Macromolecular bioscience · 2008Article
- Clodronate acts on human osteoclastic cell proliferation, differentiation and function in a bioreversible manner.Clinical cases in mineral and bone metabolism : the official journal of the Italian Society of Osteoporosis, Mineral Metabolism, and Skeletal Diseases · 2007Article
- Hyaluronan-CD44 interaction hampers migration of osteoclast-like cells by down-regulating MMP-9.The Journal of cell biology · 2002Article
- Article
- In vitro expression of proalpha1(I) collagen mRNA by human pre-osteoclastic cells.Journal of endocrinological investigation · 2000Article
- Involvement of hydrogen peroxide in the differentiation of clonal HD-11EM cells into osteoclast-like cells.Journal of cellular physiology · 1998Article
- Catecholamines modulate growth and differentiation of human preosteoclastic cells.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 1996Article
- Functional and structural interactions between osteoblastic and preosteoclastic cells in vitro.Cell and tissue research · 1995Article
- Functional estrogen receptors in a human preosteoclastic cell line.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Bidirectional regulation of osteoclast function by nitric oxide synthase isoforms.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Interactions between ipriflavone and the estrogen receptor.Calcified tissue international · 1995Article
- Establishment and characterization of stromal cell lines that support differentiation of murine hematopoietic blast cells into osteoclast-like cells.In vitro cellular & developmental biology. Animal · 1994Article
- Generation of osteoclast-inductive and osteoclastogenic cell lines from the H-2KbtsA58 transgenic mouse.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Cloning, characterization, and expression of a human calcitonin receptor from an ovarian carcinoma cell line.The Journal of clinical investigation · 1992Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studies on human osteoclast formation have been hampered by lack of a defined isolated progenitor cell population. We describe here the establishment of a human leukemic cell line (designated FLG 29.1) from bone marrow of a patient with acute monoblastic leukemia. The cultured cells are predominantly undifferentiated leukemic blasts, but addition of 12-o-tetradecanoylphorbol 13-acetate (TPA; 0.1 microM) induces irreversible differentiation into adherent, non-dividing, multinucleated cells. TPA-treated cells bear surface antigens typical of fetal osteoclasts, degrade 45Ca-labeled devitalized bone particles, display tartrate-resistant acid phosphatase in both mononuclear and multinuclear cells and receptors for calcitonin. Calcitonin increases intracellular cAMP accumulation in TPA-treated cells. TPA-treated cells show some ultrastructural features of osteoclasts as evidenced by transmission EM. These results indicate that FLG 29.1 cells may represent an osteoclast committed cell population, which upon induction with TPA acquire some morphological, phenotypical, and functional features of differentiated osteoclasts.
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