ArticleVirology1992
Identification of two new polypeptides encoded by mRNA5 of the coronavirus infectious bronchitis virus.
Article in Virology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Analysis of the complete genomic sequences of two virus subpopulations of the Australian infectious bronchitis virus vaccine VicS.Avian pathology : journal of the W.V.P.A · 2015Article
- Accessory proteins of SARS-CoV and other coronaviruses.Antiviral research · 2014Review
- Up-regulation of Mcl-1 and Bak by coronavirus infection of human, avian and animal cells modulates apoptosis and viral replication.PloS one · 2012Article
- Molecular characterization of a new species in the genus Alphacoronavirus associated with mink epizootic catarrhal gastroenteritis.The Journal of general virology · 2011Article
- The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication.Journal of virology · 2010Article
- Inhibition of protein kinase R activation and upregulation of GADD34 expression play a synergistic role in facilitating coronavirus replication by maintaining de novo protein synthesis in virus-infected cells.Journal of virology · 2009Article
- Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development.Journal of virological methods · 2009Article
- Article
- Molecular characterization of a novel coronavirus associated with epizootic catarrhal enteritis (ECE) in ferrets.Virology · 2006Article
- Neither the RNA nor the proteins of open reading frames 3a and 3b of the coronavirus infectious bronchitis virus are essential for replication.Journal of virology · 2006Article
- Genes 3 and 5 of infectious bronchitis virus are accessory protein genes.Advances in experimental medicine and biology · 2006Article
- Gene 5 of the avian coronavirus infectious bronchitis virus is not essential for replication.Journal of virology · 2005Article
- Article
- Article
- Coronavirus derived expression systems.Journal of biotechnology · 2001Review
- Induction of caspase-dependent apoptosis in cultured cells by the avian coronavirus infectious bronchitis virus.Journal of virology · 2001Article
- Translation from the 5' untranslated region (UTR) of mRNA 1 is repressed, but that from the 5' UTR of mRNA 7 is stimulated in coronavirus-infected cells.Journal of virology · 1999Article
- Identification of the leader-body junctions for the viral subgenomic mRNAs and organization of the simian hemorrhagic fever virus genome: evidence for gene duplication during arterivirus evolution.Journal of virology · 1998Article
- The molecular biology of coronaviruses.Advances in virus research · 1997Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The second smallest subgenomic messenger RNA, mRNA5, of the coronavirus infectious bronchitis virus includes in its "5' unique region" two separate open reading frames (5a and 5b), whose coding function has not so far been established, and thus it may represent a dicistronic messenger RNA. We report here that two polypeptides with the sizes expected for the 5a and 5b products can be synthesised by in vitro translation of a single artificial mRNA containing both the 5a and 5b ORFs. To establish whether these polypeptides represent genuine virus gene products, both the 5a and 5b coding sequences were expressed as bacterial fusion proteins, and these were used to raise monospecific antisera. Antisera raised against both the 5a and 5b-specific sequences recognized specifically proteins of the expected size in infectious bronchitis virus-infected chicken kidney and Vero cells, indicating that 5a and 5b do represent genuine virus genes, and suggesting that mRNA5 is indeed functionally dicistronic.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.