ArticleJournal of assisted reproduction and genetics1992
Visualization of second polar body chromosomes in fertilized and artificially activated mouse oocytes treated with okadaic acid.
Article in Journal of assisted reproduction and genetics, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.
- Rare spontaneous monochorionic dizygotic twins: a case report and a systematic review.BMC pregnancy and childbirth · 2022Pooled it
- Monochorionic Diamniotic Twins with Sex Discordance: Case Series.Diagnostics (Basel, Switzerland) · 2025Article
- Visualization of chromosomes in single human blastomeres.Journal of assisted reproduction and genetics · 1999Article
- Current progress in preimplantation genetic diagnosis.Journal of assisted reproduction and genetics · 1993Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveOur objective was to develop a new reliable method for cytogenetic analysis of the chromosome set in second polar bodies (PBs) from one-cell-stage mouse embryos.
settingThe study took place at the Reproductive Biology and Experimental Cytogenetics Laboratories.
methodsOocytes from F1 hybrid and T6/T6 mice were fertilized in vitro and artificially activated with ethanol. Zygotes, parthenogenetic embryos, and isolated second PBs were treated with 10 microM okadaic acid (OA) for 1-2 hr, further cultured in plain medium, and fixed. Chromosomal preparations were made and C-banded, and the number of chromosomes in second PBs and embryos was counted.
resultsOA-induced nuclear envelope breakdown in pronuclei as well as in second PB nuclei. Countable chromosome plates were obtained in 92-93% of second PBs treated 4-4.5 hr after activation. The T6 marker chromosome could easily be recognized in second PBs from T6/T6 mice. A haploid set of chromosomes was obtained in 18 of 19 isolated second PBs treated with OA 4-5 hr after activation.
conclusionTreatment of second PBs with OA allows visualization of the PB chromosomes. Cytogenetic analysis of the second PB and the corresponding oocyte constitutes a new approach for the study of meiotic nondisjunction in experimental cytogenetics. The chromosomal study of isolated second PBs seems to be promising for clinical preimplantation cytogenetics.
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