ArticleThe Journal of general physiology2003
Unitary Ca2+ current through mammalian cardiac and amphibian skeletal muscle ryanodine receptor Channels under near-physiological ionic conditions.
Article in The Journal of general physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 77 citations in OpenAlex.
- Modeling the spatiotemporal properties of crosstalk between RyR-mediated and IPFrontiers in cell and developmental biology · 2026Article
- Blink nadir measurements of sarcoplasmic reticulum are consistent with strong local CaBiophysical journal · 2025Article
- Quantal Properties of Voltage-Dependent CaThe Journal of membrane biology · 2024Article
- CaJournal of molecular and cellular cardiology · 2023Article
- The Cardiac Ryanodine Receptor Provides a Suitable Pathway for the Rapid Transport of Zinc (ZnCells · 2022Article
- NCX-Mediated Subcellular CaBiophysical journal · 2018Article
- A study of the mechanisms of excitation-contraction coupling in frog skeletal muscle based on measurements of [CaJournal of muscle research and cell motility · 2018Review
- Sensitization of cardiac Ca²⁺ release sites by protein kinase C signaling: evidence from action of murrayafoline A.Pflugers Archiv : European journal of physiology · 2015Article
- Sarcoplasmic reticulum K(+) (TRIC) channel does not carry essential countercurrent during Ca(2+) release.Biophysical journal · 2013Article
- Patch-clamp electrophysiology of intracellular Ca2+ channels.Cold Spring Harbor protocols · 2013Article
- Control of sarcoplasmic reticulum Ca2+ release by stochastic RyR gating within a 3D model of the cardiac dyad and importance of induction decay for CICR termination.Biophysical journal · 2013Article
- Pernicious attrition and inter-RyR2 CICR current control in cardiac muscle.Journal of molecular and cellular cardiology · 2013Review
- Article
- Ca(2+) release events in cardiac myocytes up close: insights from fast confocal imaging.PloS one · 2013Article
- Calcium spike variability in cardiac myocytes results from activation of small cohorts of ryanodine receptor 2 channels.The Journal of physiology · 2012Article
- Modelling cardiac calcium sparks in a three-dimensional reconstruction of a calcium release unit.The Journal of physiology · 2012Article
- Ryanodine receptor current amplitude controls Ca2+ sparks in cardiac muscle.Circulation research · 2012Article
- Is ryanodine receptor a calcium or magnesium channel? Roles of K+ and Mg2+ during Ca2+ release.Cell calcium · 2012Article
- Synthetic localized calcium transients directly probe signalling mechanisms in skeletal muscle.The Journal of physiology · 2012Article
- Properties of Ca2+ sparks revealed by four-dimensional confocal imaging of cardiac muscle.The Journal of general physiology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Ryanodine receptor (RyR) channels from mammalian cardiac and amphibian skeletal muscle were incorporated into planar lipid bilayers. Unitary Ca2+ currents in the SR lumen-to-cytosol direction were recorded at 0 mV in the presence of caffeine (to minimize gating fluctuations). Currents measured with 20 mM lumenal Ca2+ as exclusive charge carrier were 4.00 and 4.07 pA, respectively, and not significantly different. Currents recorded at 1-30 mM lumenal Ca2+ concentrations were attenuated by physiological [K+] (150 mM) and [Mg2+] (1 mM), in the same proportion (approximately 55%) in mammalian and amphibian channels. Two amplitudes, differing by approximately 35%, were found in amphibian channel studies, probably corresponding to alpha and beta RyR isoforms. In physiological [Mg2+], [K+], and lumenal [Ca2+] (1 mM), the Ca2+ current was just less than 0.5 pA. Comparison of this value with the Ca2+ flux underlying Ca2+ sparks suggests that sparks in mammalian cardiac and amphibian skeletal muscles are generated by opening of multiple RyR channels. Further, symmetric high concentrations of Mg2+ substantially reduced the current carried by 10 mM Ca2+ (approximately 40% at 10 mM Mg2+), suggesting that high Mg2+ may make sparks smaller by both inhibiting RyR gating and reducing unitary current.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.