ArticleThe Journal of cell biology2003
Real-time single cell analysis of Smac/DIABLO release during apoptosis.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
74 citing papers in PubMed, 150 citations in OpenAlex.
- Mitochondrial apoptosis imaged by high-throughput confocal microscopy.Scientific data · 2026Article
- The second mitochondrial activator of caspases (SMAC) regulates growth, inflammation and mitochondrial integrity in cancer cells.Cell death and differentiation · 2026Article
- Deciphering Bax Dynamics: Regulation, Retrotranslocation, and Mitochondrial Interplay in the Commitment to Apoptosis: A Narrative Review.Health science reports · 2026Article
- Stochasticity contributes to explaining minority and majority MOMP during apoptosis.Cell death & disease · 2025Article
- Mitochondrial Dysfunction is a Crucial Immune Checkpoint for Neuroinflammation and Neurodegeneration: mtDAMPs in Focus.Molecular neurobiology · 2025Review
- Thymoquinone-PLGA-PF68 Nanoparticles Induce S Phase Cell Cycle Arrest and Apoptosis, Leading to the Inhibition of Migration and Colony Formation in Tamoxifen-Resistant Breast Cancer Cells.Current molecular medicine · 2025Article
- Comprehensive pan-cancer analysis of mitochondrial outer membrane permeabilisation activity reveals positive immunomodulation and assists in identifying potential therapeutic targets for immunotherapy resistance.Clinical and translational medicine · 2024Article
- Apoptotic dysregulation mediates stem cell competition and tissue regeneration.Nature communications · 2023Article
- BAX activation in mouse retinal ganglion cells occurs in two temporally and mechanistically distinct steps.Molecular neurodegeneration · 2023Article
- BAX activation in mouse retinal ganglion cells occurs in two temporally and mechanistically distinct steps.Research square · 2023Article
- Sub-lethal signals in the mitochondrial apoptosis apparatus: pernicious by-product or physiological event?Cell death and differentiation · 2023Review
- Mitochondria supply sub-lethal signals for cytokine secretion and DNA-damage in H. pylori infection.Cell death and differentiation · 2022Article
- Cell death in skin function, inflammation, and disease.The Biochemical journal · 2022Review
- Mitochondrial dynamics regulate genome stability via control of caspase-dependent DNA damage.Developmental cell · 2022Article
- Pore formation in regulated cell death.The EMBO journal · 2020Review
- The FLAME-accelerated signalling tool (FaST) for facile parallelisation of flexible agent-based models of cell signalling.NPJ systems biology and applications · 2020Article
- Mitochondria as multifaceted regulators of cell death.Nature reviews. Molecular cell biology · 2020Review
- Povidone-iodine results in rapid killing of thymic epithelial tumour cells through cellular fixation†.Interactive cardiovascular and thoracic surgery · 2019Article
- Anastasis: recovery from the brink of cell death.Royal Society open science · 2018Review
- Apoptosis propagates through the cytoplasm as trigger waves.Science (New York, N.Y.) · 2018Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We examined the temporal and causal relationship between Smac/DIABLO release, cytochrome c (cyt-c) release, and caspase activation at the single cell level during apoptosis. Cells treated with the broad-spectrum caspase inhibitor z-VAD-fmk, caspase-3 (Casp-3)-deficient MCF-7 cells, as well as Bax-deficient DU-145 cells released Smac/DIABLO and cyt-c in response to proapoptotic agents. Real-time confocal imaging of MCF-7 cells stably expressing Smac/DIABLO-yellow fluorescent protein (YFP) revealed that the average duration of Smac/DIABLO-YFP release was greater than that of cyt-c-green fluorescent protein (GFP). However, there was no significant difference in the time to the onset of release, and both cyt-c-GFP and Smac/DIABLO-YFP release coincided with mitochondrial membrane potential depolarization. We also observed no significant differences in the Smac/DIABLO-YFP release kinetics when z-VAD-fmk-sensitive caspases were inhibited or Casp-3 was reintroduced. Simultaneous measurement of DEVDase activation and Smac/DIABLO-YFP release demonstrated that DEVDase activation occurred within 10 min of release, even in the absence of Casp-3.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.