ArticleThe Journal of cell biology2003
The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 201 papers.
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Who cites it
201 citing papers in PubMed, 376 citations in OpenAlex.
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- In vitro reconstitution defines the mechanistic basis of HSET motor activity regulation by IntraFlagellar Transport proteins.Communications biology · 2026Article
- Eg5 activity and density-driven bundling organize the human metaphase mitotic spindle independently of spindle bipolarity.bioRxiv : the preprint server for biology · 2026Article
- A coordinated kinase and phosphatase network regulates Stu2 recruitment to yeast kinetochores.The Journal of cell biology · 2025Article
- Phosphoinositide signaling at the cytoskeleton in the regulation of cell dynamics.Cell death & disease · 2025Review
- Small-Molecule Mitotic Inhibitors as Anticancer Agents: Discovery, Classification, Mechanisms of Action, and Clinical Trials.International journal of molecular sciences · 2025Review
- Dynein-driven regulation of postsynaptic membrane architecture and synaptic function.Journal of cell science · 2025Article
- Acentric chromosome congression and alignment on the metaphase plate via kinetochore-independent forces.Genetics · 2025Article
- Kinesin-5/Cut7 C-terminal tail phosphorylation is essential for microtubule sliding force and bipolar mitotic spindle assembly.Current biology : CB · 2024Article
- Patronin/CAMSAP promotes reactivation and regeneration of Drosophila quiescent neural stem cells.EMBO reports · 2023Article
- Kinesin-8-specific loop-2 controls the dual activities of the motor domain according to tubulin protofilament shape.Nature communications · 2022Article
- Article
- TEM Imaging of Membrane Choreography During Mitosis of Drosophila Tissue Culture Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Centrosome instability: when good centrosomes go bad.Cellular and molecular life sciences : CMLS · 2021Review
- Mercury interactions with selenium and sulfur and the relevance of the Se:Hg molar ratio to fish consumption advice.Environmental science and pollution research international · 2021Article
- The functions of kinesin and kinesin-related proteins in eukaryotes.Cell adhesion & migration · 2020Review
- Kinesin-13 and Kinesin-8 Function during Cell Growth and Division in the MossThe Plant cell · 2020Article
- Theory of Cytoskeletal Reorganization during Cross-Linker-Mediated Mitotic Spindle Assembly.Biophysical journal · 2019Article
- The role of Patronin in Drosophila mitosis.BMC molecular and cell biology · 2019Article
141 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Kinesins and dyneins play important roles during cell division. Using RNA interference (RNAi) to deplete individual (or combinations of) motors followed by immunofluorescence and time-lapse microscopy, we have examined the mitotic functions of cytoplasmic dynein and all 25 kinesins in Drosophila S2 cells. We show that four kinesins are involved in bipolar spindle assembly, four kinesins are involved in metaphase chromosome alignment, dynein plays a role in the metaphase-to-anaphase transition, and one kinesin is needed for cytokinesis. Functional redundancy and alternative pathways for completing mitosis were observed for many single RNAi knockdowns, and failure to complete mitosis was observed for only three kinesins. As an example, inhibition of two microtubule-depolymerizing kinesins initially produced monopolar spindles with abnormally long microtubules, but cells eventually formed bipolar spindles by an acentrosomal pole-focusing mechanism. From our phenotypic data, we construct a model for the distinct roles of molecular motors during mitosis in a single metazoan cell type.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.