ArticleThe Biochemical journal2003
Calpain facilitates GLUT4 vesicle translocation during insulin-stimulated glucose uptake in adipocytes.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 92 citations in OpenAlex.
- Type 2 diabetes candidate gene CAPN10: first, but not last.Current hypertension reports · 2008Pooled it
- Calpeptin Reduces Extracellular Vesicle Secretion and Induces Anti-Inflammatory Effects but Upregulates HO-1 and Alters Adiponectin Levels in Human Adipocytes.Journal of extracellular biology · 2026Article
- Calpain inhibition by calpeptin modulates adipocyte lipid metabolism and secretome-mediated inflammatory crosstalk with hepatocytes.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026Article
- Lack of association of the calpain-10 Indel-19 variant with chronic diseases in a Mexican population.BMC research notes · 2025Article
- Calpain and Cardiometabolic Diseases.International journal of molecular sciences · 2023Review
- Single-nucleotide polymorphisms as important risk factors of diabetes among Middle East population.Human genomics · 2022Review
- Effects of functionally diverse calpain system on immune cells.Immunologic research · 2021Review
- Serum from Jiao-Tai-Wan treated rats increases glucose consumption by 3T3-L1 adipocytes through AMPK pathway signaling.Bioscience reports · 2019Article
- Calpain-10 regulates actin dynamics by proteolysis of microtubule-associated protein 1B.Scientific reports · 2018Article
- The mTOR Complex Controls HIV Latency.Cell host & microbe · 2016Article
- Obesity and developmental delay in a patient with uniparental disomy of chromosome 2.International journal of obesity (2005) · 2016Article
- Regulation of myosin IIA and filamentous actin during insulin-stimulated glucose uptake in 3T3-L1 adipocytes.Experimental cell research · 2014Article
- Regulation of myosin light chain kinase during insulin-stimulated glucose uptake in 3T3-L1 adipocytes.PloS one · 2013Article
- Article
- Proteomic analysis reveals cellular pathways regulating carbohydrate metabolism that are modulated in primary human skeletal muscle culture due to treatment with bioactives from Artemisia dracunculus L.Journal of proteomics · 2012Article
- Mitochondrial calpain 10 is degraded by Lon protease after oxidant injury.Archives of biochemistry and biophysics · 2012Article
- Article
- Class II phosphoinositide 3-kinase regulates exocytosis of insulin granules in pancreatic beta cells.The Journal of biological chemistry · 2011Article
- Class II phosphoinositide 3-kinase C2alpha: what we learned so far.International journal of biochemistry and molecular biology · 2011Article
- Calpain chronicle--an enzyme family under multidisciplinary characterization.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2011Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Calpains are a family of non-lysosomal cysteine proteases. Recent studies have identified a member of the calpain family of proteases, calpain 10, as a putative diabetes-susceptibility gene that may be involved in the development of type 2 diabetes. Inhibition of calpain activity has been shown to reduce insulin-stimulated glucose uptake in isolated rat-muscle strips and adipocytes. In this report, we examine the mechanism by which calpain affects insulin-stimulated glucose uptake in 3T3-L1 adipocytes. Inhibition of calpain activity resulted in approx. a 60% decrease in insulin-stimulated glucose uptake. Furthermore, inhibition of calpain activity prevented the translocation of insulin-responsive glucose transporter 4 (GLUT4) vesicles to the plasma membrane, as demonstrated by fluorescent microscopy of whole cells and isolated plasma membranes; it did not, however, alter the total GLUT4 protein content. While inhibition of calpain did not affect the insulin-mediated proximal steps of the phosphoinositide 3-kinase pathway, it did prevent the insulin-stimulated cortical actin reorganization required for GLUT4 translocation. Specific inhibition of calpain 10 by antisense expression reduced insulin-stimulated GLUT4 translocation and actin reorganization. Based on these findings, we propose a role for calpain in the actin reorganization required for insulin-stimulated GLUT4 translocation to the plasma membrane in 3T3-L1 adipocytes. These studies identify calpain as a novel factor involved in GLUT4 vesicle trafficking and suggest a link between calpain activity and the development of type 2 diabetes.
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Registered trials
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