ArticlePlant physiology2003
Proteomics of Medicago truncatula seed development establishes the time frame of diverse metabolic processes related to reserve accumulation.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 260 citations in OpenAlex.
- Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations.BMC plant biology · 2025Article
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- Spatial specificity of metabolism regulation of abscisic acid-imposed seed germination inhibition in Korean pine (Pinus koraiensis sieb et zucc).Frontiers in plant science · 2024Article
- Transcriptome Shock in Developing Embryos of aInternational journal of molecular sciences · 2023Article
- OverexpressingInternational journal of molecular sciences · 2023Article
- The developmental dynamics in cool season legumes with focus on chickpea.Plant molecular biology · 2023Review
- Seed Development and Protein Accumulation Patterns in Faba Bean (Journal of agricultural and food chemistry · 2022Article
- Genome-Wide Characterization and Abiotic Stresses Expression Analysis of Annexin Family Genes in Poplar.International journal of molecular sciences · 2022Article
- Bringing New Methods to the Seed Proteomics Platform: Challenges and Perspectives.International journal of molecular sciences · 2020Review
- Temperature Regulation of Primary and Secondary Seed Dormancy inInternational journal of molecular sciences · 2020Article
- Dissecting the Seed Maturation and Germination Processes in the Non-OrthodoxInternational journal of molecular sciences · 2020Article
- Comparative Transcriptome Analysis Provides Insights into the Seed Germination in Cotton in Response to Chilling Stress.International journal of molecular sciences · 2020Article
- A proteomic analysis of peanut seed at different stages of underground development to understand the changes of seed proteins.PloS one · 2020Article
- Metabolomics: A Way Forward for Crop Improvement.Metabolites · 2019Review
- Molecular characteristics of plant UDP-arabinopyranose mutases.Glycobiology · 2019Review
- Proteome Map of Pea (International journal of molecular sciences · 2018Article
- Article
- Towards Better Understanding of Pea Seed Dormancy Using Laser Desorption/Ionization Mass Spectrometry.International journal of molecular sciences · 2017Article
- Regulation of Carbon Partitioning in the Seed of the Model LegumeFrontiers in plant science · 2017Article
- A Proteomic View on the Role of Legume Symbiotic Interactions.Frontiers in plant science · 2017Review
10 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We utilized a proteomic approach to investigate seed development in Medicago truncatula, cv Jemalong, line J5 at specific stages of seed filling corresponding to the acquisition of germination capacity and protein deposition. One hundred twenty proteins differing in kinetics of appearance were subjected to matrix-assisted laser desorption ionization time of flight mass spectrometry. These analyses provided peptide mass fingerprint data that identified 84 of them. Some of these proteins had previously been shown to accumulate during seed development in legumes (e.g. legumins, vicilins, convicilins, and lipoxygenases), confirming the validity of M. truncatula as a model for analysis of legume seed filling. The study also revealed proteins presumably involved in cell division during embryogenesis (beta-tubulin and annexin). Their abundance decreased before the accumulation of the major storage protein families, which itself occurs in a specific temporal order: vicilins (14 d after pollination [DAP]), legumins (16 DAP), and convicilins (18 DAP). Furthermore, the study showed an accumulation of enzymes of carbon metabolism (e.g. sucrose synthase, starch synthase) and of proteins involved in embryonic photosynthesis (e.g. chlorophyll a/b binding), which may play a role in providing cofactors for protein/lipid synthesis or for CO2 refixation during seed filling. Correlated with the reserve deposition phase was the accumulation of proteins associated with cell expansion (actin 7 and reversibly glycosylated polypeptide) and of components of the precursor accumulating vesicles, which give rise to a trypsin inhibitor on maturation. Finally, we revealed a differential accumulation of enzymes involved in methionine metabolism (S-adenosyl-methionine synthetase and S-adenosylhomo-cysteine hydrolase) and propose a role for these enzymes in the transition from a highly active to a quiescent state during seed development.
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