ArticlePlant physiology2003
Development of protoporphyrinogen oxidase as an efficient selection marker for Agrobacterium tumefaciens-mediated transformation of maize.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 67 citations in OpenAlex.
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- A New V361A Mutation inPlants (Basel, Switzerland) · 2023Article
- Characterization of HemY-type protoporphyrinogen IX oxidase genes from cyanobacteria and their functioning in transgenic Arabidopsis.Plant molecular biology · 2019Article
- Arabidopsis GIGANTEA negatively regulates chloroplast biogenesis and resistance to herbicide butafenacil.Plant cell reports · 2019Article
- A study on optimization of pat gene expression cassette for maize transformation.Molecular biology reports · 2019Article
- True gene-targeting events by CRISPR/Cas-induced DSB repair of the PPO locus with an ectopically integrated repair template.Scientific reports · 2018Article
- Plant Photodynamic Stress: What's New?Frontiers in plant science · 2018Review
- Advances in Maize Transformation Technologies and Development of Transgenic Maize.Frontiers in plant science · 2016Review
- Strategies to improve low copy transgenic events in Agrobacterium-mediated transformation of maize.Transgenic research · 2015Article
- Maize transformation technology development for commercial event generation.Frontiers in plant science · 2014Review
- A codon deletion confers resistance to herbicides inhibiting protoporphyrinogen oxidase.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Agriculture: the selector of improbable mutations.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- An Arabidopsis mutant that is resistant to the protoporphyrinogen oxidase inhibitor acifluorfen shows regulatory changes in tetrapyrrole biosynthesis.Molecular genetics and genomics : MGG · 2005Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this article, we report the isolation of plant protoporphyrinogen oxidase (PPO) genes and the isolation of herbicide-tolerant mutants. Subsequently, an Arabidopsis double mutant (Y426M + S305L) was used to develop a selectable marker system for Agrobacterium tumefaciens-mediated transformation of maize (Zea mays) and to obtain multiple events tolerant to the PPO family of herbicides. Maize transformants were produced via butafenacil selection using a flexible light regime to increase selection pressure. Butafenacil selection per se did not change transgene copy number distribution relative to other selectable marker systems, but the most tolerant events identified in the greenhouse were more likely to contain multiple copies of the introduced mutant PPO gene. To date, more than 2,500 independent transgenic maize events have been produced using butafenacil selection. The high frequency of A. tumefaciens-mediated transformation via PPO selection enabled us to obtain single-copy transgenic maize lines tolerant to field levels of butafenacil.
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