ArticlePlant physiology2003
Translation start sequences affect the efficiency of silencing of Agrobacterium tumefaciens T-DNA oncogenes.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 30 citations in OpenAlex.
- First Report of Crown Gall of Kiwifruit (International journal of molecular sciences · 2021Article
- Resistance analysis of cherry rootstock 'CDR-1' (Prunus mahaleb) to crown gall disease.BMC plant biology · 2020Article
- 1-(4-Amino-2-Hydroxyphenyl)Ethenone SuppressesFrontiers in microbiology · 2020Article
- Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance.Plant cell reports · 2013Article
- DNA methylation mediated control of gene expression is critical for development of crown gall tumors.PLoS genetics · 2013Article
- Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from Agrobacterium tumefaciens.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Genetically engineered plants: greener than you think.Microbial biotechnology · 2009Article
- Article
- Salicylic acid and systemic acquired resistance play a role in attenuating crown gall disease caused by Agrobacterium tumefaciens.Plant physiology · 2008Article
- Transformation of Actinidia eriantha: a potential species for functional genomics studies in Actinidia.Plant cell reports · 2006Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium tumefaciens oncogenes cause transformed plant cells to overproduce auxin and cytokinin. Two oncogenes encode enzymes that convert tryptophan to indole-3-acetic acid (auxin): iaaM (tryptophan mono-oxygenase) and iaaH (indole-3-acetamide hydrolase). A third oncogene (ipt) encodes AMP isopentenyl transferase, which produces cytokinin (isopentenyl-AMP). Inactivation of ipt and iaaM (or iaaH) abolishes tumorigenesis. Because adequate means do not exist to control crown gall, we created resistant plants by introducing transgenes designed to elicit posttranscriptional gene silencing (PTGS) of iaaM and ipt. Transgenes that elicit silencing trigger sequence-specific destruction of the inducing RNA and messenger RNAs with related sequences. Although PTGS has proven effective against a variety of target genes, we found that a much higher percentage of transgenic lines silenced iaaM than ipt, suggesting that transgene sequences influenced the effectiveness of PTGS. Sequences required for oncogene silencing included a translation start site. A transgene encoding a translatable sense-strand RNA from the 5' end of iaaM silenced the iaaM oncogene, but deletion of the translation start site abolished the ability of the transgene to silence iaaM. Silencing A. tumefaciens T-DNA oncogenes is a new and effective method to produce plants resistant to crown gall disease.
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