ArticlePlant physiology2003
Fatty acid biosynthesis in mitochondria of grasses: malonyl-coenzyme A is generated by a mitochondrial-localized acetyl-coenzyme A carboxylase.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 59 citations in OpenAlex.
- The Sapria himalayana genome provides new insights into the lifestyle of endoparasitic plants.BMC biology · 2023Article
- Two Independent Plastid accD Transfers to the Nuclear Genome of Gnetum and Other Insights on Acetyl-CoA Carboxylase Evolution in Gymnosperms.Genome biology and evolution · 2019Article
- Identification and Phenotypic Characterization ofFrontiers in plant science · 2018Article
- Acyl-lipid metabolism.The arabidopsis book · 2013Article
- Mitochondrial targeting of the Arabidopsis F1-ATPase γ-subunit via multiple compensatory and synergistic presequence motifs.The Plant cell · 2012Article
- Integration of gene-based markers in a pearl millet genetic map for identification of candidate genes underlying drought tolerance quantitative trait loci.BMC plant biology · 2012Article
- Functional analyses of two acetyl coenzyme A synthetases in the ascomycete Gibberella zeae.Eukaryotic cell · 2011Article
- Arabidopsis β-ketoacyl-[acyl carrier protein] synthase i is crucial for fatty acid synthesis and plays a role in chloroplast division and embryo development.The Plant cell · 2010Article
- Acyl-lipid metabolism.The arabidopsis book · 2010Article
- Article
- The role of plant mitochondria in the biosynthesis of coenzymes.Photosynthesis research · 2007Review
- Blue-native PAGE in plants: a tool in analysis of protein-protein interactions.Plant methods · 2005Article
- Molecular bases for sensitivity to acetyl-coenzyme A carboxylase inhibitors in black-grass.Plant physiology · 2005Article
- Annotation of genes involved in glycerolipid biosynthesis in Chlamydomonas reinhardtii: discovery of the betaine lipid synthase BTA1Cr.Eukaryotic cell · 2005Article
- Complex nested promoters control tissue-specific expression of acetyl-CoA carboxylase genes in wheat.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- New insights into the respiratory chain of plant mitochondria. Supercomplexes and a unique composition of complex II.Plant physiology · 2003Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present biochemical evidence for the occurrence of a 250-kD multifunctional acetyl-coenzyme A carboxylase in barley (Hordeum vulgare) mitochondria. Organelles from 6-d-old barley seedlings were purified by differential centrifugation and Percoll density gradient centrifugation. Upon analysis by two-dimensional Blue-native (BN)/SDS-PAGE, an abundant 250-kD protein can be visualized, which runs at 500 kD on the native gel dimension. A similar 500-kD complex is present in etioplasts from barley. The mitochondrial 250-kD protein is biotinylated as indicated by specific reaction with an antibody directed against biotin. Peptide sequence analysis by electrospray ionization tandem mass spectrometry of the 250-kD proteins from both organellar fractions revealed amino acid sequences that are 100% identical to plastidic acetyl-coenzyme A carboxylase from wheat (Triticum aestivum). The 500-kD complex was also detected in wheat mitochondria, but is absent in mitochondrial fractions from Arabidopsis. Specific acetyl-coenzyme A carboxylation activity in barley mitochondria is higher than in etioplasts, suggesting an important role of mitochondria in fatty acid biosynthesis. Functional implications are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.