ArticlePlant physiology2003
Characterization of the targeting signal of the Arabidopsis 22-kD integral peroxisomal membrane protein.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Visualisation of peroxisomes: a journey through seven decades.Histochemistry and cell biology · 2026Review
- Molecular basis of the glycosomal targeting of PEX11 and its mislocalization to mitochondrion in trypanosomes.Frontiers in cell and developmental biology · 2023Article
- Go your own way: membrane-targeting sequences.Plant physiology · 2021Article
- Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- The Arabidopsis Plastidial Glucose-6-Phosphate Transporter GPT1 is Dually Targeted to Peroxisomes via the Endoplasmic Reticulum.The Plant cell · 2020Article
- Identification of Sugarcane Host Factors Interacting with the 6K2 Protein of theInternational journal of molecular sciences · 2019Article
- Plant peroxisomal solute transporter proteins.Journal of integrative plant biology · 2019Review
- Dynamics of Peroxisome Homeostasis and Its Role in Stress Response and Signaling in Plants.Frontiers in plant science · 2019Review
- Multiple pathways for protein transport to peroxisomes.Journal of molecular biology · 2015Review
- Plant peroxisomes: biogenesis and function.The Plant cell · 2012Review
- Defining the plant peroxisomal proteome: from Arabidopsis to rice.Frontiers in plant science · 2011Article
- Biochemical characterization, mitochondrial localization, expression, and potential functions for an Arabidopsis gamma-aminobutyrate transaminase that utilizes both pyruvate and glyoxylate.Journal of experimental botany · 2009Article
- Arabidopsis PEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A cooperate in cell cycle-associated replication of peroxisomes.The Plant cell · 2008Article
- Making two organelles from one: Woronin body biogenesis by peroxisomal protein sorting.The Journal of cell biology · 2008Article
- Localization of the tomato bushy stunt virus replication protein p33 reveals a peroxisome-to-endoplasmic reticulum sorting pathway.The Plant cell · 2005Article
- AtPEX2 and AtPEX10 are targeted to peroxisomes independently of known endoplasmic reticulum trafficking routes.Plant physiology · 2005Article
- Peroxisomal membrane proteins contain common Pex19p-binding sites that are an integral part of their targeting signals.Molecular biology of the cell · 2004Article
- Specification of the peroxisome targeting signals type 1 and type 2 of plant peroxisomes by bioinformatics analyses.Plant physiology · 2004Article
- Peroxisome biogenesis and the role of protein import.Journal of cellular and molecular medicineReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using a combination of in vivo and in vitro assays, we characterized the sorting pathway and molecular targeting signal for the Arabidopsis 22-kD peroxisome membrane protein (PMP22), an integral component of the membrane of all peroxisomes in the mature plant. We show that nascent PMP22 is sorted directly from the cytosol to peroxisomes and that it is inserted into the peroxisomal boundary membrane with its N- and C-termini facing the cytosol. This direct sorting of PMP22 to peroxisomes contrasts with the indirect sorting reported previously for cottonseed (Gossypium hirsutum) ascorbate peroxidase, an integral PMP that sorts to peroxisomes via a subdomain of the endoplasmic reticulum. Thus, at least two different sorting pathways for PMPs exist in plant cells. At least four distinct regions within the N-terminal one-half of PMP22, including a positively charged domain present in most peroxisomal integral membrane-destined proteins, functions in a cooperative manner in efficient peroxisomal targeting and insertion. In addition, targeting with high fidelity to peroxisomes requires all four membrane-spanning domains in PMP22. Together, these results illustrate that the PMP22 membrane peroxisomal targeting signal is complex and that different elements within the signal may be responsible for mediating unique aspects of PMP22 biogenesis, including maintaining the solubility before membrane insertion, targeting to peroxisomes, and ensuring proper assembly in the peroxisomal boundary membrane.
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