ArticleProceedings of the National Academy of Sciences of the United States of America2003
Compensatory evolution of a precursor messenger RNA secondary structure in the Drosophila melanogaster Adh gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 61 citations in OpenAlex.
- Epistasis-Driven Evolution of the SARS-CoV-2 Secondary Structure.Journal of molecular evolution · 2022Article
- Adenine·cytosine substitutions are an alternative pathway of compensatory mutation in angiosperm ITS2.RNA (New York, N.Y.) · 2020Article
- Alternative splicing of U2AF1 reveals a shared repression mechanism for duplicated exons.Nucleic acids research · 2017Article
- Intermediate introns in nuclear genes of euglenids - are they a distinct type?BMC evolutionary biology · 2016Article
- RNA structure replaces the need for U2AF2 in splicing.Genome research · 2016Article
- The role of short RNA loops in recognition of a single-hairpin exon derived from a mammalian-wide interspersed repeat.RNA biology · 2015Article
- Strong epistatic selection on the RNA secondary structure of HIV.PLoS pathogens · 2014Article
- Distribution of conventional and nonconventional introns in tubulin (α and β) genes of euglenids.Molecular biology and evolution · 2014Article
- Evidence for widespread association of mammalian splicing and conserved long-range RNA structures.RNA (New York, N.Y.) · 2012Article
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- The role of the effective population size in compensatory evolution.Genome biology and evolution · 2011Article
- Association between colony-stimulating factor 1 receptor gene polymorphisms and asthma risk.Human genetics · 2010Article
- Article
- Role of RNA structure in regulating pre-mRNA splicing.Trends in biochemical sciences · 2010Review
- Article
- Patterns of DNA-sequence divergence between Drosophila miranda and D. pseudoobscura.Journal of molecular evolution · 2009Article
- Modulation of alternative splicing by long-range RNA structures in Drosophila.Nucleic acids research · 2009Article
- Epistatic interactions modulate the evolution of mammalian mitochondrial respiratory complex components.BMC genomics · 2009Article
- Positive and negative selection on noncoding DNA in Drosophila simulans.Molecular biology and evolution · 2008Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Evidence for the evolutionary maintenance of a hairpin structure possibly involved in intron processing had been found in intron 1 of the alcohol dehydrogenase gene (Adh) in diverse Drosophila species. In this study, the putative hairpin structure was evaluated systematically in Drosophila melanogaster by elimination of either side of the stem using site-directed mutagenesis. The effects of these mutations and the compensatory double mutant on intron splicing efficiency and ADH protein production were assayed in Drosophila melanogaster Schneider L2 cells and germ-line transformed adult flies. Mutations that disrupt the putative hairpin structure right upstream of the intron branch point were found to cause a significant reduction in both splicing efficiency and ADH protein production. In contrast, the compensatory double mutant that restores the putative hairpin structure was indistinguishable from the WT in both splicing efficiency and ADH level. It was also observed by mutational analysis that a more stable secondary structure (with a longer stem) in this intron decreases both splicing efficiency and ADH protein production. Implications for RNA secondary structure and intron evolution are discussed.
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