ArticleProceedings of the National Academy of Sciences of the United States of America2003
Ultrasensitive two-color fluorescence probes for dipole potential in phospholipid membranes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Alteration of Lipid Bilayer Electrical Potential by Phytochemicals and Synthetic Analogs: Implications for Cellular Function.Biomolecules · 2026Review
- Electronic and Steric Tuning of Molecular Acidity toward Unified Models for Excited State Proton Transfer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Look Beyond Plasma Membrane Biophysics: Revealing Considerable Variability of the Dipole Potential Between Plasma and Organelle Membranes of Living Cells.International journal of molecular sciences · 2025Article
- Proton transfer reactions: From photochemistry to biochemistry and bioenergetics.BBA advances · 2023Article
- An ω-3, but Not an ω-6 Polyunsaturated Fatty Acid Decreases Membrane Dipole Potential and Stimulates Endo-Lysosomal Escape of Penetratin.Frontiers in cell and developmental biology · 2021Article
- Natural Born Laser Dyes: Excited-State Intramolecular Proton Transfer (ESIPT) Emitters and Their Use in Random Lasing Studies.Nanomaterials (Basel, Switzerland) · 2019Article
- The dipole potential correlates with lipid raft markers in the plasma membrane of living cells.Journal of lipid research · 2017Article
- Fluorescent flavonoids for endoplasmic reticulum cell imaging.Journal of materials chemistry. B · 2016Article
- Solvatochromic fluorescent probes for recognition of human serum albumin in aqueous solution: Insights into structure-property relationship.Sensors and actuators. B, Chemical · 2016Article
- Absorption and Fluorescent Studies of 3-Hydroxychromones.Journal of fluorescence · 2015Article
- Flavone-based ESIPT ratiometric chemodosimeter for detection of cysteine in living cells.ACS applied materials & interfaces · 2014Article
- Effect of membrane tension on the electric field and dipole potential of lipid bilayer membrane.Biochimica et biophysica acta · 2011Article
- Fluorescence techniques for determination of the membrane potentials in high throughput screening.Journal of fluorescence · 2010Review
- The concept of λ-ratiometry in fluorescence sensing and imaging.Journal of fluorescence · 2010Review
- Polarity assessment of thermoresponsive poly(NIPAM-co-NtBA) copolymer films using fluorescence methods.Journal of fluorescence · 2010Article
- Excited state intramolecular proton transfer in 3-hydroxy flavone and 5-hydroxy flavone: a DFT based comparative study.Journal of molecular modeling · 2010Article
- Monitoring biophysical properties of lipid membranes by environment-sensitive fluorescent probes.Biophysical journal · 2009Review
- Dual-fluorescence probe of environment basicity (hydrogen bond accepting ability) displaying no sensitivity to polarity.Journal of fluorescence · 2009Article
- Sensing peptide-oligonucleotide interactions by a two-color fluorescence label: application to the HIV-1 nucleocapsid protein.Nucleic acids research · 2009Article
- Probing the lipid membrane dipole potential by atomic force microscopy.Biophysical journal · 2008Article
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Authors and funding
4 authors.
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Abstract
The principle of electrochromic modulation of excited-state intramolecular proton-transfer reaction was applied for the design of fluorescence probes with high two-color sensitivity to dipole potential, Psid, in phospholipid bilayers. We report on the effect of Psid variation on excitation and fluorescence spectra of two new 3-hydroxyflavone probes, which possess opposite orientations of the fluorescent moiety in the lipid bilayer. The dipole potential in the bilayer was modulated by the addition of 6-ketocholestanol or phloretin and by substitution of dimyristoyl phosphatidylcholine lipid with its ether analog 1,2-di-o-tetradecyl-sn-glycero-3-phosphocholine, and its value was estimated by the reference styryl dye 1-(3-sulfonatopropyl)-4-[beta[2-(di-n-octylamino)-6-naphthyl]vinyl]pyridinium betaine. We demonstrate that after Psid changes, the probe orienting in the bilayer similarly to the reference dye shows similar shifts in the excitation spectra, whereas the probe with the opposite orientation shows the opposite shifts. The new observation is that the response of 3-hydroxyflavone probes to Psid in excitation spectra is accompanied by and quantitatively correlated with dramatic changes of relative intensities of the two well separated emission bands that belong to the initial normal and the product tautomer forms of the excited-state intramolecular proton-transfer reaction. This provides a strong response to Psid by change in emission color.
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