ArticleMolecular and cellular biology2003
Cdk7 is required for full activation of Drosophila heat shock genes and RNA polymerase II phosphorylation in vivo.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 64 citations in OpenAlex.
- A transcription factor by any other name - function beyond DNA binding and transactivation.EMBO reports · 2026Review
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- OncogenicScience (New York, N.Y.) · 2023Article
- Enhancing HIV-1 latency reversal through regulating the elongating RNA Pol II pause-release by a small-molecule disruptor of PAF1C.Science advances · 2023Article
- The pause-initiation limit restricts transcription activation in human cells.Nature communications · 2019Article
- TFIIE orchestrates the recruitment of the TFIIH kinase module at promoter before release during transcription.Nature communications · 2019Article
- Perturbing Enhancer Activity in Cancer Therapy.Cancers · 2019Review
- The CDK7 inhibitor THZ1 alters RNA polymerase dynamics at the 5' and 3' ends of genes.Nucleic acids research · 2019Article
- Argonaute2 attenuates active transcription by limiting RNA Polymerase II elongation in Drosophila melanogaster.Scientific reports · 2018Article
- mRNA Cap Methyltransferase, RNMT-RAM, Promotes RNA Pol II-Dependent Transcription.Cell reports · 2018Article
- Is TFIIH the new Achilles heel of cancer cells?Transcription · 2018Review
- Microprocessor Recruitment to Elongating RNA Polymerase II Is Required for Differential Expression of MicroRNAs.Cell reports · 2017Article
- Functional interplay between MSL1 and CDK7 controls RNA polymerase II Ser5 phosphorylation.Nature structural & molecular biology · 2016Article
- Article
- THZ1 Reveals Roles for Cdk7 in Co-transcriptional Capping and Pausing.Molecular cell · 2015Article
- Functional role of RNA polymerase II and P70 S6 kinase in KCl withdrawal-induced cerebellar granule neuron apoptosis.The Journal of biological chemistry · 2015Article
- The metazoan-specific mediator subunit 26 (Med26) is essential for viability and is found at both active genes and pericentric heterochromatin in Drosophila melanogaster.Molecular and cellular biology · 2014Article
- Microarray analysis of cell cycle gene expression in adult human corneal endothelial cells.PloS one · 2014Article
- CDK7 regulates the mitochondrial localization of a tail-anchored proapoptotic protein, Hid.Cell reports · 2013Article
- Cyclin-dependent kinase control of the initiation-to-elongation switch of RNA polymerase II.Nature structural & molecular biology · 2012Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
TFIIH has been implicated in several fundamental cellular processes, including DNA repair, cell cycle progression, and transcription. In transcription, the helicase activity of TFIIH functions to melt promoter DNA; however, the in vivo function of the Cdk7 kinase subunit of TFIIH, which has been hypothesized to be involved in RNA polymerase II (Pol II) phosphorylation, is not clearly understood. Using temperature-sensitive and null alleles of cdk7, we have examined the role of Cdk7 in the activation of Drosophila heat shock genes. Several in vivo approaches, including polytene chromosome immunofluorescence, nuclear run-on assays, and, in particular, a protein-DNA cross-linking assay customized for adults, revealed that Cdk7 kinase activity is required for full activation of heat shock genes, promoter-proximal Pol II pausing, and Pol II-dependent chromatin decondensation. The requirement for Cdk7 occurs very early in the transcription cycle. Furthermore, we provide evidence that TFIIH associates with the elongation complex much longer than previously suspected.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.