ArticleMolecular and cellular biology2003
Comprehensive structure-function analysis of the core domain of human telomerase RNA.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Decoding RNA triple helices: identification from sequence and secondary structure.Briefings in bioinformatics · 2026Article
- Long noncoding RNAs as regulators of pediatric acute myeloid leukemia.Molecular and cellular pediatrics · 2022Review
- A structurally conserved human andProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Telomerase Inhibition by a New Synthetic Derivative of the Aporphine Alkaloid Boldine.International journal of molecular sciences · 2018Article
- New perspectives on telomerase RNA structure and function.Wiley interdisciplinary reviews. RNA · 2018Review
- Current Perspectives of Telomerase Structure and Function in Eukaryotes with Emerging Views on Telomerase in Human Parasites.International journal of molecular sciences · 2018Review
- Single-molecule FRET-Rosetta reveals RNA structural rearrangements during human telomerase catalysis.RNA (New York, N.Y.) · 2017Article
- Structure and folding of the Tetrahymena telomerase RNA pseudoknot.Nucleic acids research · 2017Article
- Structural conservation in the template/pseudoknot domain of vertebrate telomerase RNA from teleost fish to human.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Evolutionary perspectives of telomerase RNA structure and function.RNA biology · 2016Review
- Human telomerase reverse transcriptase binds to a pre-organized hTR in vivo exposing its template.Nucleic acids research · 2016Article
- Refined secondary-structure models of the core of yeast and human telomerase RNAs directed by SHAPE.RNA (New York, N.Y.) · 2015Article
- Human telomerase specialization for repeat synthesis by unique handling of primer-template duplex.The EMBO journal · 2014Article
- RNA connectivity requirements between conserved elements in the core of the yeast telomerase RNP.The EMBO journal · 2013Article
- Pyrimidine motif triple helix in the Kluyveromyces lactis telomerase RNA pseudoknot is essential for function in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells.Cellular & molecular biology letters · 2012Article
- Telomere lengthening and other functions of telomerase.Acta naturae · 2012Article
- It all comes together at the ends: telomerase structure, function, and biogenesis.Mutation research · 2012Review
- Architecture of human telomerase RNA.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Molecular mechanisms of long noncoding RNAs.Molecular cell · 2011Review
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Authors and funding
3 authors.
Funding
Abstract
Telomerase is a cellular reverse transcriptase that uses part of its integral RNA (called TER) as the template to synthesize telomeric DNA repeats. Vertebrate TERs are thought to share a conserved, highly structured core domain that includes the templating sequence and a pseudoknot, but not all features of the predicted core structure have been verified directly or shown to affect telomerase enzymatic activity. Here, we report a systematic mutational analysis of the core domain (residues 1 to 210) of human telomerase RNA (hTER). Our data confirm that optimal hTER activity requires the integrity of four short helices (P2a.1, P2a, P2b, and P3) which create the proposed pseudoknot and that features of both the primary sequence and secondary structure in P2b and P3 contribute to optimal function. At least part of the long-range P1 pairing is also required, despite the lack of a known P1 counterpart in rodent TERs. Among the predicted single-stranded regions, we found that J2b/3, portions of J2a/3, and residues in and around the template make sequence-specific contributions to telomerase function. Additionally, we provide evidence that naturally occurring hTER sequence polymorphisms found in some patients with aplastic anemia can inhibit telomerase activity by disrupting critical structures within the hTER core domain.
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