ArticleMolecular and cellular biology2003
CpG methylation of DNA restricts prereplication complex assembly in Xenopus egg extracts.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 63 citations in OpenAlex.
- Article
- The genetic landscape of origins of replication in P. falciparum.Nucleic acids research · 2024Article
- Where and when to start: Regulating DNA replication origin activity in eukaryotic genomes.Nucleus (Austin, Tex.) · 2023Review
- Neural network and kinetic modelling of human genome replication reveal replication origin locations and strengths.PLoS computational biology · 2023Article
- Origins of DNA replication in eukaryotes.Molecular cell · 2023Review
- Mobile origin-licensing factors confer resistance to conflicts with RNA polymerase.Cell reports · 2022Article
- Chromatin structure restricts origin utilization when quiescent cells re-enter the cell cycle.Nucleic acids research · 2021Article
- MCM interference during licensing of DNA replication in Xenopus egg extracts-Possible Role of a C-terminal region of MCM3.Cell cycle (Georgetown, Tex.) · 2018Article
- The High-Affinity Interaction between ORC and DNA that Is Required for Replication Licensing Is Inhibited by 2-Arylquinolin-4-Amines.Cell chemical biology · 2017Article
- Deciphering DNA replication dynamics in eukaryotic cell populations in relation with their averaged chromatin conformations.Scientific reports · 2016Article
- Replication landscape of the human genome.Nature communications · 2016Article
- How MCM loading and spreading specify eukaryotic DNA replication initiation sites.F1000Research · 2016Review
- Dynamic loading and redistribution of the Mcm2-7 helicase complex through the cell cycle.The EMBO journal · 2015Article
- RecQ4 promotes the conversion of the pre-initiation complex at a site-specific origin for DNA unwinding in Xenopus egg extracts.Cell cycle (Georgetown, Tex.) · 2015Article
- Replication timing and its emergence from stochastic processes.Trends in genetics : TIG · 2012Review
- How dormant origins promote complete genome replication.Trends in biochemical sciences · 2011Review
- Initiation of DNA replication: functional and evolutionary aspects.Annals of botany · 2011Review
- Article
- Arabidopsis thaliana chromosome 4 replicates in two phases that correlate with chromatin state.PLoS genetics · 2010Article
- Mathematical modelling of eukaryotic DNA replication.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In a Xenopus egg replication system, the origin recognition complex (ORC) does not bind to CpG methylated DNA and DNA replication is inhibited. Insertion of low density CpG DNA of at least 1.2 kb into methylated plasmids rescues both replication and ORC binding. Using this pseudo-origin, we find that ORC binding is restricted to low-CpG-density DNA; however, MCM is loaded onto both weakly and highly methylated DNA and occupies at least approximately 2 kb of DNA. Replication initiates coincident with MCM, and even the most distally bound MCM is associated with sites of replication initiation. These results suggest that in metazoans MCM is loaded onto and initiates replication over a large region distant from ORC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.