ArticleMolecular and cellular biology2003
A dominant-negative thyroid hormone receptor blocks amphibian metamorphosis by retaining corepressors at target genes.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 125 citations in OpenAlex.
- Understanding thyroid hormone receptor function in controlling developmental timing and rate in Xenopus: A journey from biochemistry and molecular biology to genetics.General and comparative endocrinology · 2026Review
- The dual function model revisited by thyroid hormone receptor knockouts: Unliganded state controlling the initiation of adult organ development while liganded state ensuring larval tissue resorption during metamorphosis.Developmental biology · 2026Review
- Review
- Thyroid hormone receptor subtype-specific function in controlling organ-specific developmental timing and rate duringFrontiers in endocrinology · 2025Review
- Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis.bioRxiv : the preprint server for biology · 2024Article
- Functions and Mechanism of Thyroid Hormone Receptor Action During Amphibian Development.Endocrinology · 2024Review
- Intestinal remodeling during Xenopus metamorphosis as a model for studying thyroid hormone signaling and adult organogenesis.Molecular and cellular endocrinology · 2024Article
- Thyroid hormone regulates proximodistal patterning in fin rays.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Comparative Analysis of Transcriptome Profiles Reveals Distinct and Organ-Dependent Genomic and Nongenomic Actions of Thyroid Hormone inThyroid : official journal of the American Thyroid Association · 2023Article
- Cell cycle activation in thyroid hormone-induced apoptosis and stem cell development duringFrontiers in endocrinology · 2023Review
- Steroid-receptor coactivator complexes in thyroid hormone-regulation of Xenopus metamorphosis.Vitamins and hormones · 2023Article
- Essential and subtype-dependent function of thyroid hormone receptors during Xenopus metamorphosis.Vitamins and hormones · 2023Review
- Upregulation of proto-oncogene ski by thyroid hormone in the intestine and tail during Xenopus metamorphosis.General and comparative endocrinology · 2022Article
- Review
- Thyroid hormone regulates abrupt skin morphogenesis during zebrafish postembryonic development.Developmental biology · 2021Article
- Life Without Thyroid Hormone Receptor.Endocrinology · 2021Review
- Article
- The development of adult intestinal stem cells: Insights from studies on thyroid hormone-dependent anuran metamorphosis.Vitamins and hormones · 2021Article
- Thyroid hormone directly activates mitochondrial fission process 1 (Mtfp1) gene transcription during adult intestinal stem cell development and proliferation in Xenopus tropicalis.General and comparative endocrinology · 2020Article
- Thyroid Hormone Induces DNA Demethylation in Xenopus Tadpole Brain.Endocrinology · 2020Article
15 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The total dependence of amphibian metamorphosis on thyroid hormone (T(3)) provides a unique vertebrate model for studying the molecular mechanism of T(3) receptor (TR) function in vivo. In vitro transcription and developmental expression studies have led to a dual function model for TR in amphibian development, i.e., TRs act as transcriptional repressors in premetamorphic tadpoles and as activators during metamorphosis. We examined molecular mechanisms of TR action in T3-induced metamorphosis by using dominant-negative receptors (dnTR) ubiquitously expressed in transgenic Xenopus laevis. We showed that T(3)-induced activation of T(3) target genes and morphological changes are blocked in dnTR transgenic animals. By using chromatin immunoprecipitation, we show that dnTR bound to target promoters, which led to retention of corepressors and continued histone deacetylation in the presence of T(3). These results thus provide direct in vivo evidence for the first time for a molecular mechanism of altering gene expression by a dnTR. The correlation between dnTR-mediated gene repression and inhibition of metamorphosis also supports a key aspect of the dual function model for TR in development: during T(3)-induced metamorphosis, TR functions as an activator via release of corepressors and promotion of histone acetylation and gene activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.