ArticleMolecular and cellular biology2003
Role of metalloprotease disintegrin ADAM12 in determination of quiescent reserve cells during myogenic differentiation in vitro.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 72 citations in OpenAlex.
- Grb2/Sos1 signaling regulates the number of reserve cells in C2C12 cell culture.In vitro cellular & developmental biology. Animal · 2026Article
- Functions of 'A disintegrin and metalloproteases (ADAMs)' in the mammalian nervous system.Cellular and molecular life sciences : CMLS · 2019Review
- Proteomic screening identifies the zonula occludens protein ZO-1 as a new partner for ADAM12 in invadopodia-like structures.Oncotarget · 2018Article
- The development of skeletal muscle hypertrophy through resistance training: the role of muscle damage and muscle protein synthesis.European journal of applied physiology · 2018Review
- Genome Editing and Muscle Stem Cells as a Therapeutic Tool for Muscular Dystrophies.Current stem cell reports · 2017Review
- Muscle Satellite Cells: Exploring the Basic Biology to Rule Them.Stem cells international · 2016Review
- Intrinsic and extrinsic mechanisms regulating satellite cell function.Development (Cambridge, England) · 2015Review
- Chronic Prosopis glandulosa treatment blunts neutrophil infiltration and enhances muscle repair after contusion injury.Nutrients · 2015Article
- The Disintegrin and Metalloprotease ADAM12 Is Associated with TGF-β-Induced Epithelial to Mesenchymal Transition.PloS one · 2015Article
- Muscle stem cells at a glance.Journal of cell science · 2014Article
- Identification of genomic regions associated with feed efficiency in Nelore cattle.BMC genetics · 2014Article
- Human skeletal muscle-derived CD133(+) cells form functional satellite cells after intramuscular transplantation in immunodeficient host mice.Molecular therapy : the journal of the American Society of Gene Therapy · 2014Article
- A disintegrin and metalloproteinase-12 (ADAM12): function, roles in disease progression, and clinical implications.Biochimica et biophysica acta · 2013Review
- Signal-dependent fra-2 regulation in skeletal muscle reserve and satellite cells.Cell death & disease · 2013Article
- Identification of ILK as a new partner of the ADAM12 disintegrin and metalloprotease in cell adhesion and survival.Molecular biology of the cell · 2012Article
- Metalloprotease-disintegrin ADAM12 expression is regulated by Notch signaling via microRNA-29.The Journal of biological chemistry · 2011Article
- Rb1 gene inactivation expands satellite cell and postnatal myoblast pools.The Journal of biological chemistry · 2011Article
- The role of Delta-like 1 shedding in muscle cell self-renewal and differentiation.Journal of cell science · 2008Article
- The ADAM metalloproteinases.Molecular aspects of medicine · 2008Review
- Proteolytic processing of delta-like 1 by ADAM proteases.The Journal of biological chemistry · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Skeletal myoblasts grown in vitro and induced to differentiate either form differentiated multinucleated myotubes or give rise to quiescent, undifferentiated "reserve cells" that share several characteristics with muscle satellite cells. The mechanism of determination of reserve cells is poorly understood. We find that the expression level of the metalloprotease disintegrin ADAM12 is much higher in proliferating C2C12 myoblasts and in reserve cells than in myotubes. Inhibition of ADAM12 expression in differentiating C2C12 cultures by small interfering RNA is accompanied by lower expression levels of both quiescence markers (retinoblastoma-related protein p130 and cell cycle inhibitor p27) and differentiation markers (myogenin and integrin alpha7A isoform). Overexpression of ADAM12 in C2C12 cells under conditions that promote cell cycle progression leads to upregulation of p130 and p27, cell cycle arrest, and downregulation of MyoD. Thus, enhanced expression of ADAM12 induces a quiescence-like phenotype and does not stimulate differentiation. We also show that the region extending from the disintegrin to the transmembrane domain of ADAM12 and containing cell adhesion activity as well as the cytoplasmic domain of ADAM12 are required for ADAM12-mediated cell cycle arrest, while the metalloprotease domain is not essential. Our results suggest that ADAM12-mediated adhesion and/or signaling may play a role in determination of the pool of reserve cells during myoblast differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.