ArticleMolecular biology of the cell2003
XRad17 is required for the activation of XChk1 but not XCds1 during checkpoint signaling in Xenopus.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Energetically equivalent structural transitions in the Rad17-Rad9-Hus1-Rad1-Rhino complex underlie the sequential progression from activation through maintenance to inactivation of the ATR-dependent DNA damage response.Nucleic acids research · 2026Article
- APE1 senses DNA single-strand breaks for repair and signaling.Nucleic acids research · 2020Article
- Distinct roles of XRCC1 in genome integrity in Xenopus egg extracts.The Biochemical journal · 2019Article
- APE2 promotes DNA damage response pathway from a single-strand break.Nucleic acids research · 2018Article
- Cip29 is phosphorylated following activation of the DNA damage response in Xenopus egg extracts.PloS one · 2017Article
- The Mre11-Rad50-Nbs1 (MRN) complex has a specific role in the activation of Chk1 in response to stalled replication forks.Molecular biology of the cell · 2013Article
- Role of replication protein A as sensor in activation of the S-phase checkpoint in Xenopus egg extracts.Nucleic acids research · 2012Article
- Rad17 plays a central role in establishment of the interaction between TopBP1 and the Rad9-Hus1-Rad1 complex at stalled replication forks.Molecular biology of the cell · 2010Article
- TopBP1 and DNA polymerase-alpha directly recruit the 9-1-1 complex to stalled DNA replication forks.The Journal of cell biology · 2009Article
- Analyzing the ATR-mediated checkpoint using Xenopus egg extracts.Methods (San Diego, Calif.) · 2007Article
- Deregulated replication licensing causes DNA fragmentation consistent with head-to-tail fork collision.Molecular cell · 2006Article
- Phosphorylation of Xenopus Rad1 and Hus1 defines a readout for ATR activation that is independent of Claspin and the Rad9 carboxy terminus.Molecular biology of the cell · 2006Article
- Xenopus Cds1 is regulated by DNA-dependent protein kinase and ATR during the cell cycle checkpoint response to double-stranded DNA ends.Molecular and cellular biology · 2004Article
- Absence of BLM leads to accumulation of chromosomal DNA breaks during both unperturbed and disrupted S phases.The Journal of cell biology · 2004Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The DNA damage/replication checkpoints act by sensing the presence of damaged DNA or stalled replication forks and initiate signaling pathways that arrest cell cycle progression. Here we report the cloning and characterization of Xenopus orthologues of the RFCand PCNA-related checkpoint proteins. XRad17 shares regions of homology with the five subunits of Replication factor C. XRad9, XRad1, and XHus1 (components of the 9-1-1 complex) all show homology to the DNA polymerase processivity factor PCNA. We demonstrate that these proteins associate with chromatin and are phosphorylated when replication is inhibited by aphidicolin. Phosphorylation of X9-1-1 is caffeine sensitive, but the chromatin association of XRad17 and the X9-1-1 complex after replication block is unaffected by caffeine. This suggests that the X9-1-1 complex can associate with chromatin independently of XAtm/XAtr activity. We further demonstrate that XRad17 is essential for the chromatin binding and checkpoint-dependent phosphorylation of X9-1-1 and for the activation of XChk1 when the replication checkpoint is induced by aphidicolin. XRad17 is not, however, required for the activation of XCds1 in response to dsDNA ends.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.