ArticleMolecular biology of the cell2003
Histone hyperacetylation in mitosis prevents sister chromatid separation and produces chromosome segregation defects.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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81 citing papers in PubMed, 182 citations in OpenAlex.
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- Single-nucleosome imaging unveils that condensins and nucleosome-nucleosome interactions differentially constrain chromatin to organize mitotic chromosomes.Nature communications · 2024Article
- Manipulation of the nuclear envelope-associated protein SLAP during mammalian brain development affects cortical lamination and exploratory behavior.Biology open · 2024Article
- Histone deacetylation and cytosine methylation compartmentalize heterochromatic regions in the genome organization ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Ability of a selfish B chromosome to evade genome elimination in the jewel wasp, Nasonia vitripennis.Heredity · 2023Article
- Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase.The EMBO journal · 2023Article
- The material properties of mitotic chromosomes.Current opinion in structural biology · 2023Review
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- Whole-Genome Duplication and Genome Instability in Cancer Cells: Double the Trouble.International journal of molecular sciences · 2023Review
- SIRT1 regulates mitotic catastrophe via autophagy and BubR1 signaling.Molecular and cellular biochemistry · 2022Article
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- Unravelling the Role of Epigenetic Modifications in Development and Reproduction of Angiosperms: A Critical Appraisal.Frontiers in genetics · 2022Review
- Histone deacetylase inhibition leads to regulatory histone mark alterations and impairs meiosis in oocytes.Epigenetics & chromatin · 2021Article
- ReducedCancers · 2021Article
- Histone modifications form a cell-type-specific chromosomal bar code that persists through the cell cycle.Scientific reports · 2021Article
21 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Posttranslational modifications of core histones contribute to driving changes in chromatin conformation and compaction. Herein, we investigated the role of histone deacetylation on the mitotic process by inhibiting histone deacetylases shortly before mitosis in human primary fibroblasts. Cells entering mitosis with hyperacetylated histones displayed altered chromatin conformation associated with decreased reactivity to the anti-Ser 10 phospho H3 antibody, increased recruitment of protein phosphatase 1-delta on mitotic chromosomes, and depletion of heterochromatin protein 1 from the centromeric heterochromatin. Inhibition of histone deacetylation before mitosis produced defective chromosome condensation and impaired mitotic progression in living cells, suggesting that improper chromosome condensation may induce mitotic checkpoint activation. In situ hybridization analysis on anaphase cells demonstrated the presence of chromatin bridges, which were caused by persisting cohesion along sister chromatid arms after centromere separation. Thus, the presence of hyperacetylated chromatin during mitosis impairs proper chromosome condensation during the pre-anaphase stages, resulting in poor sister chromatid resolution. Lagging chromosomes consisting of single or paired sisters were also induced by the presence of hyperacetylated histones, indicating that the less constrained centromeric organization associated with heterochromatin protein 1 depletion may promote the attachment of kinetochores to microtubules coming from both poles.
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