ArticleMolecular biology of the cell2003
Alpha4beta1 integrin/ligand interaction inhibits alpha5beta1-induced stress fibers and focal adhesions via down-regulation of RhoA and induces melanoma cell migration.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Mechanistic insights into the adsorption and bioactivity of fibronectin on surfaces with varying chemistries by a combination of experimental strategies and molecular simulations.Bioactive materials · 2021Article
- α4/α9 Integrins Coordinate Epithelial Cell Migration Through Local Suppression of MAP Kinase Signaling Pathways.Frontiers in cell and developmental biology · 2021Article
- Nanoscale Tuning of VCAM-1 Determines VLA-4-Dependent Melanoma Cell Plasticity on RGD Motifs.Molecular cancer research : MCR · 2018Article
- Distinct signaling mechanisms regulate migration in unconfined versus confined spaces.The Journal of cell biology · 2013Article
- Ovarian cancer spheroids use myosin-generated force to clear the mesothelium.Cancer discovery · 2011Article
- Integrin-linked kinase regulates integrin signaling in human trabecular meshwork cells.Investigative ophthalmology & visual science · 2011Article
- Heparin II domain of fibronectin mediates contractility through an alpha4beta1 co-signaling pathway.Experimental cell research · 2010Article
- Tumor suppressor protein SMAR1 modulates the roughness of cell surface: combined AFM and SEM study.BMC cancer · 2009Article
- Identification of the active site in the heparin II domain of fibronectin that increases outflow facility in cultured monkey anterior segments.Investigative ophthalmology & visual science · 2009Article
- Inadequate activation of the GTPase RhoA contributes to the lack of fibronectin matrix assembly in von Hippel-Lindau protein-defective renal cancer cells.The Journal of biological chemistry · 2008Article
- Automated time-lapse microscopy and high-resolution tracking of cell migration.Cytotechnology · 2006Article
- Effects of constitutively active GTPases on fibroblast behavior.Cellular and molecular life sciences : CMLS · 2006Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied the function of the Hep III fibronectin domain in the cytoskeletal response initiated by alpha5beta1 integrin-mediated adhesion. Melanoma cells formed stress fibers and focal adhesions on the RGD-containing FNIII7-10 fragment. Coimmobilization of FNIII4-5, a fragment spanning Hep III and containing the alpha4beta1 ligand H2 with FNIII7-10, or addition of soluble FNIII4-5 to cells preattached to FNIII7-10, inhibited stress fibers and induced cytoplasmic protrusions. This effect involved alpha4beta1 since: 1) mutations in H2 reverted the inhibition; 2) other alpha4beta1 ligands (CS-1, VCAM-1), an anti-alpha4 mAb, or alpha4 expression in HeLa cells inhibited stress fibers. This activity was apparently cryptic in fibronectin or large fibronectin fragments, but exposed upon proteolytic degradation. Indeed purified peptic fragments containing H2, inhibited stress fibers when mixed with FNIII7-10 or fibronectin. RhoA activation with LPA or transfection with V14RhoA reverted the inhibitory effect and induced stress fibers on FNIII7-10+FNIII4-5. Furthermore, addition of alpha4beta1 ligands to FNIII7-10, down-regulated RhoA and activated p190RhoGAP, which localized to cytoplasmic protrusions. alpha4beta1/ligand interaction induced cell migration, monitored by video microscopy and wound healing assays. These data indicate that alpha4beta1 provides an antagonistic signal to alpha5beta1 by interfering with the RhoA activation pathway and this leads to melanoma cell migration.
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